
- SpeciesReactivityHuman
- SpecificityDetectshumanCarbonicAnhydrase IV/CA4indirectELISAsandWesternblots.IndirectELISAsandWesternblots,approximately20% cross‑reactivitywithrecombinantmouseCA4isobserved andlessthan5% cross-reactivitywithrecombinanthumanCA1,2,3,8,9,10,12,and13isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanCarbonicAnhydrase IV/CA4
Ala19-Lys283
Accession#P22748 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLSeebelow
- SimpleWestern10µg/mLSeebelow
- Immunohistochemistry5-15µg/mLImmersionfixedparaffin-embeddedsectionsofhumancolon
- Immunoprecipitation25µg/mLConditionedcellculturemediumspikedwithRecombinantHumanCarbonicAnhydrase IV(Catalog #2186-CA),seeouravailableWesternblotdetectionantibodies
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Hewett-Emmett,D.andR.E.Tashian(1996)Mol.Phylogenet.Evol.5:50.
- Chandrashekar,J.etal.(2009)Science326:443.
- Rebello,G.etal.(2004)Proc.Natl.Acad.Sci.USA101:6617.
- EntrezGeneIDs:762(Human);12351(Mouse)
- AlternateNames:CA4;CAIV;CA-IV;Car4;CarbonatedehydrataseIV;carbonicanhydrase4;CarbonicAnhydraseIV;carbonicanhydraseIVRP17;carbonicdehydrataseIV;EC4.2.1.1;retinitispigmentosa17(autosomaldominant);RP17
Background:
CarbonicAnhydrase(CA)catalyzesthereversIBLereactionofCO2+H2O=HCO3-+H+,whichisfundamentaltomanyprocessessuchasrespiration,renaltubularacidificationandboneresorption(1).Topicsina CAmeeting(6thInternationalConferenceontheCAs,June 20‑25,2003,Slovakia)rangedfromtheuseofCAsasMarkersfortumorandhypoxiaintheclinic,asanutritionalsupplementinmilk,andasatoolforCO2removalandmosquitocontrolinindustry.CA4isaGPI‑anchoredmembraneenzymeexpressedontheluminalsurfacesofpulmonary(andcertainother)capillariesandofproximalrenaltubules.ItfunctionsastheprincipalCO2tastesensor(2).Inaddition,ageneticmutation(Arg14toTrpinthesignalpeptide)ofCA4wasfoundtocosegregatewiththeRP17formofretinitispigmentosaintwolargefamiliesandwasnotfoundin36unaffectedfamilymembersor100controls(3).
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