
- SpeciesReactivityHuman
- SpecificityDetectshumanEDNRBindirectELISAsandWesternblots.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanEDNRB
Glu27-Lys101
Accession#P24530 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLSeebelow
- FlowCytometry2.5µg/106cellsSeebelow
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- Immunocytochemistry5-15µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:EndothelinReceptorTypeB
- EntrezGeneIDs:1910(Human);13618(Mouse);50672(Rat)
- AlternateNames:ABCDS;EDNRB;endothelinBreceptor;Endothelinreceptornon-selectivetype;endothelinreceptortypeB;ETB;ET-B;ETBR;ET-BR;ETRB;HSCR;HSCR2;WS4A
Background:
EDNRB(EndothelinBReceptor)isamemberofthebeta-familyofrhodopsinreceptors.Itbindsendothelin1,2,and3andisfoundonendothelialcellswhereitmediatesvasodilation.MaturehumanEDNRBisa7-transmembraneglycoproteinthatis416 aminoacids(aa)inlength.Itcontainsa75 aaN-terminalextracellularregion(aa 27-101),anda44 aaC-terminalcytoplasmicdomain.TherearethreeEDNRBvariantsthataffectaa 27-101.Oneshowsa90 aaN-terminalextension,asecondshowsthesame90 aaN-terminalsubstitutioncoupledwithadeletionofaa 268-398,andathirdshowsproteolyticcleavagebetweenArg64-Ser65.Overaa 27-101,humanEDNRBshares67% and97% aasequenceidentitywithmouseandcanineEDNRB,respectively.
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