
- Species ReactivityHuman
- SpecificityDetects human EDNRB in direct ELISAs and Western blots.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human EDNRB
Glu27-Lys101
Accession # P24530 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot1 µg/mLSee below
- Flow Cytometry2.5 µg/106 cellsSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- Immunocytochemistry5-15 µg/mLSee below
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Endothelin Receptor Type B
- Entrez Gene IDs:1910 (Human); 13618 (Mouse); 50672 (Rat)
- Alternate Names:ABCDS; EDNRB; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A
Background:
EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.
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