
- SpeciesReactivityMouse
- SpecificityDetectsXCL1/LymphotactinindirectELISAsandWesternblots. IndirectELISAs, lessthan5% cross-reactivitywithrecombinanthuman(rh) GROalpha,rhGRObeta,rhGROgamma,andrecombinantmouse MCP-5isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinantmouseXCL1/Lymphotactin
- FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot0.1µg/mLRecombinantMouseXCL1/Lymphotactin(Catalog#486-LT)
- Immunohistochemistry5-15µg/mLPerfusionfixedfrozensectionsofmousesmallintestine(Peyer"spatch)andthymus
- NeutralizationMeasuredbyitsABIlitytoneutralizeXCL1/Lymphotactin-inducedchemotaxisintheBaF3mousepro‑BcelllinetransfectedwithhumanXCR1.TheNeutralizationDose(ND50)istypically5-15 µg/mLinthepresenceof0.15 µg/mLRecombinantMouseXCL1/Lymphotactin.
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Kelner,G.etal.(1994)Science266:1395.
- Hedrick,J.A.etal.(1997)J.Immunol.158:1533.
- LongName:Chemokine[CMotif]Ligand1
- EntrezGeneIDs:6375(Human);16963(Mouse)
- AlternateNames:ATAC;ATACSmall-inducIBLecytokineC1;Cmotifchemokine1;chemokine(Cmotif)ligand1;CytokineSCM-1;LPTNSCM-1-alpha;LTNXCchemokineligand1;Lymphotactin;lymphotaxin;SCM-1alpha;SCM-1;SCM1A;SCM-1a;SCYC1SCM1;singlecysteinemotif1a;smallinduciblecytokinesubfamilyC,member1(lymphotactin);XCL1
Background:
Mouselymphotactin(Lptn)anditshumanhomologue(alsonamedhumanSCM-1andATAC)belongtotheCorgammasubfamilyofchemokines.TheCchemokineslacktwo(the1stand3rd)ofthefourinvariantcysteineresiduesnormallyfoundintheCCandCXC chemokinesandhaveanextendedcarboxyterminus.Mouselymphotactinencodesa114aminoacidresidueprecursorproteinwitha21aminoacidresiduepredictedsignalpeptide.Theexpressionoflymphotactinisrestrictedtoactivatedmousepro-T cellsandtoactivated,classIMHCrestrictedT cells.Thegeneforlymphotactinhasbeenmappedtochromosome 1inbothhumanandmouse.
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