
- SpeciesReactivityHuman,Mouse,Rat
- SpecificityDetectshuman,mouse, andrat NEDD8indirectELISAsandWesternblots.IndirectELISAsandWesternblots,lessthan
2% cross‑reactivitywithrecombinanthumanNEDD9isobserved. - SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanNEDD8
Leu2-Gly77
Accession#Q15843 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot2µg/mLSeebelow
- Immunohistochemistry5-15µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:NeuralPrecursorCellExpressedDevelopmentallyDown-RegulatedProtein8
- EntrezGeneIDs:4738(Human);18002(Mouse);25490(Rat)
- AlternateNames:FLJ43224;MGC104393;MGC125896;MGC125897;NEDD8;NEDD-8;Neddylin;Neuralprecursorcellexpresseddevelopmentallydown-regulatedprotein8;neuralprecursorcellexpressed,developmentallydown-regulated8;Rub1;Ubiquitin-likeproteinNedd8
Background:
NEDD8(Neuralprecursorcell-expresseddevelopmentallydown-regulatedgene 8;alsoRub1)isa6-8 kDamemberoftheubiquitinfamilyofproteins.Itisexpressedinstriatedmuscle,andviaitsC-terminalGly,formscovalentbondswithcullinfamilyproteins.Thisconjugationgeneratesubiquitinligaseactivitythatregulatescellcycleproteins.Humanpro-NEDD8is81 aminoacids(aa)inlength.Itcontainsoneubiquitin-likedomain(aa 1-65),anda4-5 aaC-terminalpropeptidethatiscleavedtoexposeaC-terminalGlyresiduethatisusedtogenerateaGly-Lysintermolecularbond.MaturehumanNEDD8shows100% aasequence identitytoNEDD8frombothmouseandcanine.
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