
- SpeciesReactivityHuman
- SpecificityDetectshumanLKB1/STK11indirectELISAsandWesternblots.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanLKB1/STK11
Met1-Gln433
Accession#Q15831 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot2µg/mLSeebelow
- Immunohistochemistry5-15µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:Serine/threonineProteinKinaseLKB1
- EntrezGeneIDs:6794(Human);20869(Mouse);314621(Rat)
- AlternateNames:EC2.7.11.1;LKB1serine/threoninekinase11(Peutz-Jegherssyndrome);LKB1;PJSpolarization-relatedproteinLKB1;PJS;RenalcarcinomaantigenNY-REN-19;serine/threoninekinase11;serine/threonine-proteinkinase11;Serine/threonine-proteinkinaseLKB1;STK11
Background:
LKB1(LiverkinaseB1;alsoSTK11/Ser/Thr-proteinkinase11andNY-REN19)isa55kDaintracellularmemberoftheLKB1subfamily,CAMKSer/Thrproteinkinasefamilyofmolecules.Itiswidelyexpressed,beingparticularlyinvestigatedinsmallintestinalcolumnarepitheliumandhepatocytes.STK11isassociatedwithawidevarietyoffunctions,includingtheinitiationofapoptosisthroughbindingtop53,theregulationofTGF-betasignalingthroughthecreationofaSMAD4-LIP1-STK11complex,andthegenerationofanepithelialpolarizedphenotypeviacytoskeletalremodeling.Schematically,itwouldappearthatSTK11acts,atleastinpart,throughitsabilitytophosphorylatemultipleAMPK-relatedkinases,aswellasAMPKitself.HumanSTK11is433aminoacids(aa)inlength.ItpotentiallycontainsathreeaapropeptideatitsC-terminus.Thematuresegment(aa1-430)possessesonelargeproteinkinasedomain(aa49-309)plusatleastfourutilizedphosphorylationsites. PhosphorylationonSer428promotestheabilityofLKB1tosuppressG361cellgrowth.PalmitoylationissuggestedtooccuronCys418.Therearethreealternativesplicevariants.Eitherindividuallyorincombination,theyinvolveaninsertionofnineaaafterTyr126anda34aasubstitutionforaa371-433.Full-lengthhumanSTK11shares90%aasequenceidentitywithmouseSTK11.
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