
- SpeciesReactivityMouse
- SpecificityDetectsmouseMMP-12indirectELISAsandWesternblots.IndirectELISAs,approximately20%cross-reactivitywithrecombinanthumanMMP‑12isobserved,andlessthan5%cross-reactivitywithrecombinantmouse(rm)MMP-3,rmMMP-8,andrmMMP-10isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseMMP-12
Ala18-Cys462
Accession#EDL24933 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot2µg/mLSeebelow
- Immunoprecipitation25µg/mLConditionedcellculturemediumspikedwithRecombinantMouseMMP‑12(Catalog #3467‑MP),seeouravailableWesternblotdetectionantibodies
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:MatrixMetalloproteinase12
- EntrezGeneIDs:4321(Human);17381(Mouse)
- AlternateNames:EC3.4.24;hME;HMEEC3.4.24.65;Macrophageelastase;macrophagemetalloelastase;matrixmetallopeptidase12(macrophageelastase);matrixmetalloproteinase12(macrophageelastase);Matrixmetalloproteinase-12;ME;MGC138506;MME;MMP12;MMP-12
Background:
Matrixmetalloproteinases(MMPs)areafamilyofzincandcalciumdependentendopeptidaseswiththecombinedabilitytodegradeallthecomponentsoftheextracellularmatrix.MMP-12(macrophageelastase)isfoundinmacrophagesanditsexpressioninmonocytescanbeinducedbycytokinessuchasGM-CSFandCD40signaling.Inadditiontoelastin,MMP-12candegradeabroadspectrumofsubstrates,includingtypeIVcollagen,fibronectin,laminin,vitronectin,proteoglycans,chondroitinsulfate,myelinbasicprotein,alpha1-antitrypsin,andplasminogen.ItcanalsoactivateMMP-2andMMP-3.MMP-12isrequiredformacrophage-mediatedproteolysisandmatrixinvasioninmice.MMP-12isproposedtohaveadirectroleinthepathogenesisofaorticaneurysmsandinthedevelopmentofpulmonaryemphysemathatresultsfromchronicinhalationofcigarettesmoke.Structurally,thepro-MMP-12consistsoffollowingdomains:aprodomain,acatalyticdomaincontainingthezinc-bindingsite,andaC-terminalhemopexin-likedomain.TherecombinantmouseMMP-12correspondstotheproformthatcanbeactivated.
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