
- SpeciesReactivityHuman
- SpecificityDetectshumanCytokeratin19indirectELISAsandWesternblots.IndirectELISAs, lessthan 10%cross-reactivitywithrecombinanthuman Cytokeratin14 isobserved.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanCytokeratin19
Thr2-Leu400
Accession#P08727 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot0.1µg/mLSeebelow
- SimpleWestern1µg/mLSeebelow
- Immunocytochemistry5-15µg/mLSeebelow
- ReconstitutionSterilePBStoafinalconcentrationof0.2mg/mL.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- EntrezGeneIDs:3880(Human);16669(Mouse)
- AlternateNames:CK19;CK-19;Cytokeratin19;Cytokeratin-19,40-kDakeratinintermediatefilament;EndoC;K19;K19cytokeratin-19;K1CS;keratin19;keratin,typeIcytoskeletal19;keratin,typeI,40-kd;keratin-19;Krt19;MGC15366
Background:
Cytokeratin19(Keratin,typeIcytoskeletal19;alsoKRT-19,CK19andKeratin-19)isa40-45kDa,acidicClassIkeratinmemberoftheintermediatefilamentfamilyofproteins.Individualkeratinsarealwaysexpressedintandemwithasecondkeratin,andthesearefoundinallepithelialcells.TheclassIKRT-19heterodimerizes/polymerizeswith50-52kDaclassIIKRT-8(plusKRT-5and-7)toform8-10nmfilamentsinepidermalstemcells,secretorygland(sweat;mammary;bileduct)simpleepithelium,andneuroendocrineepidermalMerkelcells.ItmayrepresentaviableMarkerforskinstemcells.Inskin,Cytokeratin19formsfilamentsinthefetalepithelium,andthenprogressivelydecreaseswithage,beingvirtuallyabsentbyage17.HumanCytokeratin19is400aminoacids(aa)inlength.ItcontainsanN-terminal"head"region(aa1-79)andasubsequent"rod"region(aa80-387),butisabsentatypicalC-terminaltailregion.Cytokeratin19possessesatleast5utilizedphosphorylationsitesplusoneacetylatedLysresidue.Basedonotherkeratins,andthepresenceofanAspatposition238,theremaybecaspasecleavage-generatedisoforms.FulllengthhumanCytokeratin19(aa2-400)shares82%aasequenceidentitywithmouseCytokeratin19.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>


暂无品牌分类