
- SpeciesReactivityHuman
- SpecificityDetectshumanGLI-2indirectELISAsandWesternblots.IndirectELISAsandWesternblots, approximately25%cross-reactivitywithrecombinantmouseGLI-2and5%cross-reactivitywithrecombinanthuman(rh)GLI-1andrhGLI-3isobserved.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinanthumanGLI-2
Ala2-Glu91
Accession#BAA25668 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot0.1µg/mLRecombinantHumanGLI‑2
- Immunohistochemistry5-15µg/mLImmersionfixedparaffin-embeddedsectionsofhumanskin,basalcellcarcinoma,andprostatecancertissue
- ChromatinImmunoprecipitation(ChIP)5µg/5x106cellsSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:GLI-KruppelfamilymemberGLI2
- EntrezGeneIDs:2736(Human);14633(Mouse);304729(Rat)
- AlternateNames:GLIfamilyzincfinger2;GLI2;GLI-2;GLI-KruppelfamilymemberGLI2;glioma-associatedoncogenefamilyzincfinger2;HPE9taxhelperprotein1;oncogeneGLI2;taxhelperprotein2;Taxhelperprotein;tax-responsiveelement-2holdingprotein;tax-responsiveelement-25-bpsequencebindingprotein;THP;THP1;THP2;zincfingerproteinGLI2
Background:
GLI-2isa166kDabiopotentialtranscriptionregulatorofthehedgehogsignalingpathway.Itcontains5conservedtandemC2H2zincfingerdomainsthatareflankedbyarepressiondomainattheN-terminalregionandanactivationdomainintheC-terminalregion.AtleastfourisoformsthatdifferintheirN-orC-terminalregionshavebeendescribed.TranscriptionalactivityofGLI-2isregulatedbyproteolyticprocessinganddegradation.Withintheregionusedasimmunogen,humanandmouseGLI-2share88%aminoacidsequencehomology.
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