- SpeciesReactivityHuman
- SpecificityDetectshumanECM-1indirectELISAsandWesternblots.IndirectELISAs,lessthan10%cross-reactivitywithrecombinantmouseECM‑1isobserved.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanECM-1
Ala20-Glu540
Accession#AAH23505 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLSeebelow
- SimpleWestern10µg/mLSeebelow
- Immunohistochemistry5-15µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Chan,I.(2004)Exp.Dermatol.29:52.
- Smits,P.etal.(1997)Genomics45:487.
- Bhalerao,J.etal.(1995)J.Biol.Chem270:16385.
- Smits,P.etal.(2000)J.Invest.Dermatol.114:718.
- Mongiat,M.etal.(2003)J.Biol.Chem.278:17491.
- Han,Z.etal.(2001)FASEBJ.15:988.
- Wang,L.etal.(2003)CancerLett.200:57.
- Hamada,T.etal.(2003)J.Invest.Dermatol.120:345.
- Oyama,N.etal.(2004)J.Clin.Invest.113:1550.
- Fujimoto,N.etal.(2005)Biochem.Biophys.Res.Commun.333:1327.
- LongName:ExtracellularMatrixProtein1
- EntrezGeneIDs:1893(Human);13601(Mouse);116662(Rat)
- AlternateNames:ECM1;extracellularmatrixprotein1;p85;SecretoryComponentGlycoprotein;SecretoryComponentP85
Background:
Extracellularmatrixprotein-1(ECM-1)isan85kDa,secretedglycoproteinimportantinconnectivetissueorganization(1‑3).Ofthreeidentifiedsplicevariantsthe540 aminoacid(aa)form,ECM-1a,isthemostwidelyexpressed,withthehighestexpressionintheplacentaandheart(2).ECM-1b(415aa)isfoundonlyintonsilandassociatedwithsuprabasalkeratinocytes(2, 4).SinceECM-1bexpressionisdifferentiation-dependent,aroleinterminalkeratinocytedifferentiationhasbeensuggested(4).ECM-1c(559 aa)accountsforapproximately15%ofskinECM-1(5).HumanECM-1acontainsa19aasignalpeptideanda521aasecretedportionthatincludesanN-terminalproline-rich,cysteine-freeregion,twotandemrepeatdomains,andaC-terminaldomain.TherearesixrepeatsofaCC(X7‑10)Cmotif(x = any aa)withinthetandemrepeatandC‑terminaldomains.Thesemotifsareinvolvedinligandbindingtomembersofthealbuminfamily,andareexpectedtoformtwo(inECM-1b)orthree(inECM-1a)“doubleloop”structures(2).MaturehumanECM-1ashows69%,71%,72%,and76%aaidentitywithcorrespondingisoformsofmouse,rat,canine,and bovineECM-1,respectively.ECM-1isover-expressedinmanymalignantepithelialtumorsandhasdemonstratedangiogenicactivity(6, 7).AvarietyofECM-1mutations,mainlywithinthefirsttandemrepeat,areconsideredcausativeoflipoidproteinosis,aconditionshowingthickenedandirregularextracellularmatrixwithinconnectivetissue(8).Intheautoimmuneconditionlichensclerosis,auto-antibodiesmainlyrecognizethesecondtandemrepeatortheC-terminusofECM-1(9).Thesedomainsalsobindtheextracellularmatrixmoleculesfibulin-1andperlecan(5, 10).ThephenotypesoflipoidproteinosisandlichensclerosissupportaroleforECM-1asa“BIOLOGicalglue”inthedermis(1).
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