
- Species ReactivityHuman
- SpecificityDetects human MMP-12 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse
MMP-12 is observed, and less than 2% cross-reactivity with recombinant human (rh) MMP-1 and rhMMP-3 is observed. - SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenMouse myeloma cell line NS0-derived recombinant human MMP-12
Leu17-Cys470
Accession # P39900 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot2 µg/mLSee below
- Immunohistochemistry5-15 µg/mLSee below
- Immunoprecipitation25 µg/mLConditioned cell culture medium spiked with Recombinant Human MMP‑12 (Catalog # 917‑MP), see our available Western blot detection antibodies
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- S.D. Shapiro et al. (2004) in Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) pp.540 - 544, Academic Press, San Diego.
- Long Name:Matrix Metalloproteinase 12
- Entrez Gene IDs:4321 (Human); 17381 (Mouse)
- Alternate Names:EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12
Background:
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-12 (macrophage elastase) is found in macrophages and its expression in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling (1). In addition to elastin, MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein,alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. MMP-12 is required for macrophage‑mediated proteolysis and matrix invasion in mice. MMP-12 is proposed to have a direct role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. Structurally, the pro MMP-12 consists of following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The rhMMP-12 corresponds to the pro form that can be activated by autocatalysis under the conditions described above.
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