
- Species ReactivityHuman
- SpecificityDetects human Cathepsin S in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human Cathepsin A, -B, -C, -D, E, L, V, and -X/Z/P is observed.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenMouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331
Accession # P25774 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
- LabelBiotin
- Western Blot0.1 µg/mLRecombinant Human Cathepsin S (Catalog # 1183-CY)
- Immunohistochemistry5-15 µg/mLSee below
- Human Cathepsin S Sandwich ImmunoassayReagent
- ELISA Capture (Matched Antibody Pair)0.2-0.8 µg/mLHuman Cathepsin S Antibody (Catalog # AF1183)
- ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mLHuman Cathepsin S Biotinylated Antibody (Catalog # BAF1183)
- ELISA StandardRecombinant Human Cathepsin S Protein, CF (Catalog # 1183-CY)
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
- Entrez Gene IDs:1520 (Human)
- Alternate Names:Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886
Background:
Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.
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