
- SpeciesReactivityHuman
- SpecificityDetectshumanIL-17BinWesternblotsandELISAs.InWesternblots, approximately25%cross-reactivitywithrecombinantmouseIL‑17Bisobserved andnocross-reactivitywithrecombinanthuman (rh) IL‑17,rhIL-17C,rhIL-17EorrhIL-17Fisobserved.Insandwichimmunoassays,lessthan0.5%cross-reactivitywithrmIL-17B,andnocross-reactivitywithrhIL-17C,rhIL-17D,rhIL-17E,rhIL-17F,orrhIL-17isobserved.
- SourceMonoclonalMouseIgG2BClone#174113
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinanthumanIL-17B
Gln21-Phe180
Accession#Q9UHF5 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLRecombinantHumanIL‑17B(Catalog#1248-IB)
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- IntracellularStainingbyFlowCytometry2.5µg/106cellsSeebelow
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Aggarwal,S.andA.L.Gurney(2002)J.Leukoc.Biol.71:1.
- Moseley,T.A.etal.(2003)Cytokine&GrowthFactorRev.14:155.
- Hymowitz,S.G.etal.(2001)EMBOJ.20:5332.
- Haudenschild,D.etal.(2002)J.Biol.Chem.277:4309.
- Shi,Y.etal.(2000)J.Biol.Chem.275:19167.
- Li,H.etal.(2000)Proc.Natl.Acad.Sci.USA97:773.
- Moore,E.E.etal.(2002)Neuromuscul.Disord.12:141.
- LongName:Interleukin17B
- EntrezGeneIDs:27190(Human);56069(Mouse)
- AlternateNames:CytokineZcyto7;cytokine-likeproteinZCYTO7;IL17B;IL-17B;IL-17Binterleukin-17beta;IL20;IL-20interleukin-17B;interleukin17B;interleukin20;Interleukin-20;MGC138900;MGC138901;neuronalinterleukin-17relatedfactor;Neuronalinterleukin-17-relatedfactor;NIRF;ZCYTO7interleukin-20
Background:
TheInterleukin17(IL-17)familyproteins,comprisingsixmembers(IL-17,IL-17BthroughIL-17F),aresecreted,structurallyrelatedproteinsthatshareaconservedcystine-knotfoldneartheC-terminus,buthaveconsiderablesequencedivergenceattheN-terminus(1, 2).WiththeexceptionofIL-17B,whichexistsasanon‑covalentlylinkeddimer,allIL-17familymembersaredisulfide-linkeddimers(3).IL-17familyproteinsarepro‑inflammatorycytokinesthatinducelocalcytokineproductionandareinvolvedintheregulationofimmunefunctions(1, 2).Tworeceptors(IL‑17 R,andIL-17BR),whichareactivatedbyIL-17familymembers,havebeenidentified.Inaddition,atleastthreeadditionalorphantypeItransmembranereceptorswithhomologytoIL-17R,includingIL-17RL(IL-17RC),IL-17RD,andIL‑17 RE,havealsobeenreported(1‑4).HumanIL-17BCDNAencodesa180aaproteinwithaputative20aasignalpeptide(5,6).HumanandmouseIL-17Bshare88%aminoacidsequenceidentity.AmongIL-17familymembers,IL-17BismostcloselyrelatedtoIL-17D,sharing27%aasequencehomology.IL-17Bisexpressedhighlyinspinalcord,andatlowerlevelsinbrain,kidney,lung,smallintestine,prostate,testes,pancreas,adrenalglandandtrachea(5‑7).ExpressionofIL-17Bhasalsobeendetectedinchondrocytesinarticularcartilage(2).IL-17BbindstheIL-17BreceptorbutnotIL-17RandexhibitsbioactivitiesdistinctfromthoseofIL-17(5,6).
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