
- Species ReactivityMouse
- SpecificityDetects mouse CCL12/MCP‑5 in ELISAs and Western blots.
- SourceMonoclonal Rat IgG2A Clone # 63205
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant mouse CCL12/MCP-5
Gly23-Gly104
Accession # Q62401.1 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- Endotoxin Level<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- Western Blot1 µg/mLRecombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5)
- Mouse CCL12/MCP-5 Sandwich ImmunoassayReagent
- ELISA Capture (Matched Antibody Pair)2-8 µg/mLMouse CCL12/MCP‑5 Antibody (Catalog # MAB428)
- ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mLMouse CCL12/MCP‑5 Biotinylated Antibody (Catalog # BAF428)
- ELISA StandardRecombinant Mouse CCL12/MCP-5 Protein (Catalog # 428-P5)
- NeutralizationMeasured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse CCL12/MCP‑5.
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
- Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.
- Entrez Gene IDs:20293 (Mouse)
- Alternate Names:C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12
Background:
CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>


暂无品牌分类