
- SpeciesReactivityMouse
- SpecificityDetectsmouseCCL12/MCP‑5inELISAsand Westernblots.
- SourceMonoclonalRatIgG2AClone#63205
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinantmouseCCL12/MCP-5
Gly23-Gly104
Accession#Q62401.1 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot1µg/mLRecombinantMouseCCL12/MCP‑5(Catalog#428-P5)
- MouseCCL12/MCP-5SandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLMouseCCL12/MCP‑5Antibody(Catalog#MAB428)
- ELISADetection(MatchedAntibodyPair)0.1-0.4µg/mLMouseCCL12/MCP‑5BiotinylatedAntibody(Catalog#BAF428)
- ELISAStandardRecombinantMouseCCL12/MCP-5Protein(Catalog#428-P5)
- NeutralizationMeasuredbyitsABIlitytoneutralizeCCL12/MCP‑5-inducedchemotaxisintheBaF3mousepro‑BcelllinetransfectedwithhumanCCR2A.TheNeutralizationDose(ND50)istypically0.5-2.5μg/mLinthepresenceof10 ng/mLRecombinantMouseCCL12/MCP‑5.
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Jia, G.Q.et al.(1996)J.Exp.Med.184:1939.
- Sarafi,M.N.etal.(1997)J.Exp.Med.185:99.
- EntrezGeneIDs:20293(Mouse)
- AlternateNames:C-Cmotifchemokine12;CCL12;chemokine(C-Cmotif)ligand12;MCP5;MCP-5;Scya12
Background:
CCL12isaclonedmouseCCchemokinemostcloselyrelatedtohumanMCP-1(66%aminoacid(aa)sequenceidentityinthematureprotein).MouseCCL12encodesa104aaresidueprecursorproteinwitha22aaresiduepredictedhydrophobicsignalsequencethatiscleavedtogeneratea82aaresiduematureprotein.CCL12isexpressedconstitutivelyinthethymusandlymphnodes.Underinflammatoryconditions,CCL12expressionisalsoinducedinactivatedmacrophagesandmastcells.ThegeneformouseMCP-1hasbeenmappedtotheCCchemokineclusteronchromosome11.RecombinantCCL12hasbeenshowntobeapotentchemoattractantformonocytesandlymphocytesbutnotneutrophils.Athighconcentrations,CCL12willalsochemoattracteosinophils.CCL12hasbeenfoundtobeafunctionalligandforCCR2butnotCCR1,CCR3,orCCR5.
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