
- Species ReactivityMouse
- SpecificityDetects mouse Sema-4F in ELISAs.
- SourceMonoclonal Rat IgG2A Clone # 780225
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Sema-4F
Arg41-Gly667
Accession # Q9Z123 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Flow Cytometry2.5 µL/106 cellsSee below
- Immunohistochemistry8-25 µg/mLSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Entrez Gene IDs:10505 (Human); 20355 (Mouse); 29745 (Rat)
- Alternate Names:M-SEMA; m-Sema-M; PRO2353; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F; Sema M; Sema W; SEMA4F; SEMAM; Semaphorin 4F; semaphorin-4F; semaphorin-M; semaphorin-W; SEMAW; SEMAWsemaphorin M
Background:
Semaphorin 4F (Sema4F; also Sema W) is a 90-100 kDa, class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively.
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