- Species ReactivityHuman
- SpecificityDetects human Lymphotoxin‑ alpha /TNF‑ betain ELISAs. In sandwich immunoassays, approximately 10% cross‑reactivity with recombinant mouse TNF‑ betais observed and no cross-reactivity with bovine FGF acidic, recombinant human (rh) GM‑CSF, rhIL‑1 alpha, rhIL‑1 beta, rhIL‑2, rhIL‑3, rhIL‑4, rhIL‑6, rhIL‑8, rhTNF‑ alpha, rhPDGF, rpPDGF, rhTGF‑ beta 1, recombinant porcine (rp) TGF‑ beta 1, rpTGF‑ beta 1.2, or rpTGF‑ beta 2 is observed.
- SourceMonoclonal Mouse IgG2A Clone # 5807
- PurificationProtein A or G purified from ascites
- ImmunogenE. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
- FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- Endotoxin Level<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">
- LabelUnconjugated
- Human Lymphotoxin-alpha /TNF-betaSandwich ImmunoassayReagent
- ELISA Capture (Matched Antibody Pair)2-8 µg/mLHuman Lymphotoxin‑ alpha /TNF‑ betaAntibody (Catalog # MAB621)
- ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mLHuman Lymphotoxin‑ alpha /TNF‑ betaBiotinylated Antibody (Catalog # BAF211)
- ELISA StandardRecombinant Human Lymphotoxin-alpha/TNF-beta Protein (Catalog # 211-TBB)
- NeutralizationMeasured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ betaand actinomycin D.
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Tumor Necrosis Factor beta
- Entrez Gene IDs:4049 (Human); 16992 (Mouse)
- Alternate Names:LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
Background:
Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alphaand TNF-alphashare approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alphacan exist as a type II membrane protein, TNF-beta /LT-alphapossesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alphais also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-betaare closely linked within the major histocompatibility complex.
TNF-beta /LT-alphais expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alphahas been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alphaand TNF-alphaare mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alphahas also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alphashare approximately 74% homology in their amino acid sequence and exhibit cross-species activity.
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