
- Species ReactivityHuman
- SpecificityDetects human IGFBP-4 in ELISAs and Western blots.
- SourceMonoclonal Mouse IgG1 Clone # 82314
- PurificationProtein A or G purified from ascites
- ImmunogenMouse myeloma cell line NS0-derived recombinant human IGFBP‑4
Asp22-Glu258
Accession # AAA62670 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- Endotoxin Level<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- Western Blot1 µg/mLRecombinant Human IGFBP‑4 (Catalog # 804-GB)
- Human IGFBP-4 Sandwich ImmunoassayReagent
- ELISA Capture (Matched Antibody Pair)2-8 µg/mLHuman IGFBP‑4 Antibody (Catalog # MAB8041)
- ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mLHuman IGFBP‑4 Biotinylated Antibody (Catalog # BAF804)
- ELISA StandardRecombinant Human IGFBP-4 Protein, CF (Catalog # 804-GB)
- NeutralizationMeasured by its ability to neutralize IGFBP‑4 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 20‑80 µg/mL in the presence of 0.3 µg/mL Recombinant Human IGFBP‑4 and 14 ng/mL Recombinant Human IGF‑II.
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Jones, J.I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
- Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
- Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.
- Long Name:Insulin-like Growth Factor Binding Protein 4
- Entrez Gene IDs:3487 (Human); 16010 (Mouse)
- Alternate Names:BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4
Background:
Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, 4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.
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