
- SpeciesReactivityHuman
- SpecificityDetectshumanIGFBP-4inELISAsandWesternblots.
- SourceMonoclonalMouseIgG1Clone#82314
- PurificationProteinAorGpurifiedfromascites
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanIGFBP‑4
Asp22-Glu258
Accession#AAA62670 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot1µg/mLRecombinantHumanIGFBP‑4(Catalog#804-GB)
- HumanIGFBP-4SandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLHumanIGFBP‑4Antibody(Catalog#MAB8041)
- ELISADetection(MatchedAntibodyPair)0.1-0.4µg/mLHumanIGFBP‑4BiotinylatedAntibody(Catalog#BAF804)
- ELISAStandardRecombinantHumanIGFBP-4Protein,CF(Catalog#804-GB)
- NeutralizationMeasuredbyitsABIlitytoneutralizeIGFBP‑4inhibitionofIGF‑II-dependentproliferationintheMCF‑7humanbreastcancercellline.Karey,K.P.etal.(1988)CancerResearch48:4083.TheNeutralizationDose(ND50)istypically20‑80 µg/mLinthepresenceof0.3 µg/mLRecombinantHumanIGFBP‑4and14 ng/mLRecombinantHumanIGF‑II.
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Jones,J.I.andD.R.Clemons(1995)EndocrineRev.16:3.
- Kelly,K.M.etal.(1996)Intl.J.Biochem.CellBiol.28:619.
- Kim,H-S.etal.(1997)Proc.Natl.Acad.Sci.USA94:12981.
- LongName:Insulin-likeGrowthFactorBindingProtein4
- EntrezGeneIDs:3487(Human);16010(Mouse)
- AlternateNames:BP-4;HT29-IGFBP;IBP4insulin-likegrowthfactor-bindingprotein4;IGF-bindingprotein4;IGFBP4;IGFBP-4;IGFBP-4IBP-4;insulin-likegrowthfactorbindingprotein4
Background:
HumanIGFbindingprotein4(IGFBP-4)wasisolatedfromhumanplasmabasedonitsabilitytobindimmobilizedIGF-I.HumanIGFBP-4CDNAencodesa258aminoacid(aa)residueprecursorproteinwithapredicted21aaresiduesignalpeptidethatisprocessedtogeneratethe237aaresiduematurehumanIGFBP-4.ThehumanIGFBP-4containsapotentialN-linkedglycosylationsiteandsharesapproximately90%aminoacidsequenceidentitywithboththemouseandratIGFBP-4.AccordingtothenomenclatureofIGFBPsdefinedatthe4thInternationalSymposiumofIGFs(1997,Tokyo),sixhigh-affinityIGFbindingproteins(IGFBP-1,-2,-3,4,-5,-6),andfourIGFBP-relatedproteins(IGFBPr-1,-2,-3,-4)havebeenidentified.AllIGFBPshaveahighcysteinecontentandshareconservedcysteineresiduesthatareclusteredintheamino-andcarboxy-terminalthirdofthemolecule.IGFBPshavebeenshowntoeitherinhibitorenhancetheBIOLOGicalactivitiesofIGF,oractinanIGF-independentmanner.Post-translationalmodificationofIGFBPs,includingphosphorylationandproteolysis,havebeenshowntomodifytheaffinitiesofthebindingproteinsforIGFandmayindirectlyregulateIGFactions.
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