- SpeciesReactivityHuman
- SpecificityDetectshumanandmouseHGFR/c-METwhenphosphorylatedatY1003.
- SourcePolyclonalRabbitIgG
- PurificationAntigenAffinity-purified
- ImmunogenPhosphopeptidecontaininghumanHGFRY1003site
- FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLSeebelow
- Immunohistochemistry5-15µg/mLImmersionfixedparaffin-embeddedsectionsofhumanbreastcancertissue
- Immunocytochemistry5-15µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
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- Ponzetto,C.etal.(1994)Cell77:261.
- Jeffers,M.etal.(1997)Mol.Cell.Biol.17:799.
- Orian-Rousseau,V.etal.(2002)GenesDev.16:3074.
- Klosek,S.K.etal.(2005)Biochem.Biophys.Res.Commun.336:408.
- Jo,M.etal.(2000)J.Biol.Chem.275:8806.
- Wang,X.etal.(2002)Mol.Cell9:411.
- Trusolino,L.etal.(2001)Cell107:643.
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- LongName:HepatocyteGrowthFactorReceptor
- EntrezGeneIDs:4233(Human);17295(Mouse)
- AlternateNames:AUTS9;cMET;c-MET;EC2.7.10;EC2.7.10.1;hepatocytegrowthfactorreceptor;HGFR;HGFreceptor;HGF/SFreceptor;HGFR;Met(c-Met);metproto-oncogene(hepatocytegrowthfactorreceptor);metproto-oncogenetyrosinekinase;MET;oncogeneMET;Proto-oncogenec-Met;RCCP2;Scatterfactorreceptor;SFreceptor;Tyrosine-proteinkinaseMet
Background:
HGFR,alsoknownasMet(fromN-methyl-N’-nitro-N-nitrosoguanidineinduced),isaglycosylatedreceptortyrosinekinasethatplaysacentralroleinepithelialmorphogenesisandcancerdevelopment.HGFRissynthesizedasasinglechainprecursorwhichundergoescotranslationalproteolyticcleavage.ThisgeneratesamatureHGFRthatisadisulfide-linkeddimercomposedofa50kDaextracellularalphachainanda145kDatransmembraNEBetachain(1,2).Theextracellulardomain(ECD)containsasevenbladedbeta-propellersemadomain,acysteine-richPSI/MRS,andfourIg-likeE-setdomains,whilethecytoplasmicregionincludesthetyrosinekinasedomain(3,4).ProteolysisandalternatesplicinggenerateadditionalformsofhumanHGFRwhicheitherlackofthekinasedomain,consistofsecretedextracellulardomains,oraredeficientinproteolyticseparationofthealphaandbetachains(5-7).Thesemadomain,whichisformedbyboththealphaandbetachainsofHGFR,mediatesbothligandbindingandreceptordimerization(3,8).Ligand-inducedtyrosinephosphorylationinthecytoplasmicregionactivatesthekinasedomainandprovidesdockingsitesformultipleSH2-containingmolecules(9,10).HGFstimulationinducesHGFRdownregulationviainternalizationandproteasome-dependentdegradation(11).Intheabsenceofligand,HGFRformsnoncovalentcomplexeswithavarietyofmembraneproteinsincludingCD44v6,CD151,EGFR,Fas,Integrinalpha6/beta4,PlexinsB1,2,3,andMSPR/Ron(12-19).Ligationofonecomplexcomponenttriggersactivationoftheother,followedbycooperativesignalingeffects(12-19).Formationofsomeoftheseheteromericcomplexesisarequirementforepithelialcellmorphogenesisandtumorcellinvasion(12,16,17).ParacrineinductionofepithelialcellscatteringandbranchingtubulogenesisresultsfromthestimulationofHGFRonundifferentiatedepitheliumbyHGFreleasedfromneighboringmesenchymalcells(20).Geneticpolymorphisms,chromosomaltranslocation,overexpression,andadditionalsplicingandproteolyticcleavageofHGFRhavebeendescribedinawiderangeofcancers(1).WithintheECD,humanHGFRshares86%-88%aasequenceidentitywithcanine,mouse,andratHGFR.
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