
- Species ReactivityHuman, Mouse, Rat
- SpecificityDetects human, mouse, and rat Calcineurin B in Western blots.
- SourcePolyclonal Rabbit IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human Calcineurin B
Accession # P63098 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot0.25 µg/mLSee below
- Simple Western2.5 µg/mLSee below
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
- Zeng H. et al. (2001) Cell 107:617.
- Okamura, H. et al. (2000) Molecular Cell 6:539.
- Liu, J. et al. (1992) Biochemistry 31:3896.
- Molkentin, J.D. (2000) Circulation Research 87:731.
- Sanquer, S.A. et al. (2004) Transplantation 77:854.
- Entrez Gene IDs:5534 (Human); 19058 (Mouse); 29748 (Rat)
- Alternate Names:Calcineurin B; calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa)
Background:
Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>


暂无品牌分类