
- SpeciesReactivityMouse
- SpecificityDetectsmousegp130indirectELISAsandWesternblots.IndirectELISAs, approximately10%cross-reactivitywithrecombinanthumangp130isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmousegp130
Gln23-Glu617
Accession#Q6PDI9 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot0.1µg/mLRecombinantMousegp130FcChimera(Catalog#468-MG)
- FlowCytometry2.5µg/106cellsM1mousemyeloidleukemiacellline
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- NeutralizationMeasuredbyitsABIlitytoneutralizeIL‑6-inducedinhibitionofproliferationintheM1mousemyeloidleukemiacellline.Saito,T.etal.(1991)J.Immunol.147:168.TheNeutralizationDose(ND50)istypically0.3-1.0 µg/mLinthepresenceof20 ng/mLRecombinantHumanIL‑6.
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Narazaki, M.et al.(1993)Blood82:1120.
- Taga, T.andT. Kishimoto(1997)Annu.Rev.Immunol.15:797.
- LongName:Glycoprotein130
- EntrezGeneIDs:3572(Human);16195(Mouse);25205(Rat)
- AlternateNames:CD130antigen;CD130;CDw130;DKFZp564F053;gp130oftherheumatoidarthritisantigenicpeptide-bearingsolubleform;gp130;IL-6receptorsubunitbeta;IL-6Rsubunitbeta;IL-6RB;IL-6R-beta;IL6ST;interleukin6signaltransducer(gp130,oncostatinMreceptor);interleukinreceptorbetachain;interleukin-6receptorsubunitbeta;Interleukin-6signaltransducer;Membraneglycoprotein130;membraneglycoproteingp130;Oncostatin-Mreceptorsubunitalpha
Background:
Gp130,thecommonsignaltransducingreceptorcomponentsharedbythefunctionalreceptorcomplexesoftheIL-6familyofcytokines,belongstotheclass Icytokinereceptorfamily.BindingofIL-6(IL-11)toeitherthemembrane-anchoredorsolubleIL-6 R(IL-11 R)initiatestheassociationofIL-6 R(IL-11 R)withgp130whichthenundergoeshomo-dimerizationandsignaltransduction.WithotherIL-6familycytokines,suchasLIFandOSM,signaltransductionistriggeredbythehetero-dimerizationofgp130andLIF RorOSM R.
Gp130isexpressedinallorgansexamined.Solublegp130,whichapparentlyariseseitherfromproteolyticcleavageofthemembrane-boundreceptororfromalternativesplicing,hasbeendetectedinhumanserum. Thein vivofunctionsofsolublegp130arenotclearlyunderstood.Inin vitroexperiments,naturalorrecombinantsolublegp130hasbeenshowntohaveinhibitoryeffectsonOSMandCNTFactivities.
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