
- SpeciesReactivityHuman
- SpecificityDetectshumanTRANCE/TNFSF11/RANKLindirectELISAsandWesternblots.IndirectELISAs,lessthan30% cross‑reactivitywithrecombinantmouseTRANCEisobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenS.frugiperdainsectovariancelllineSf21-derivedrecombinanthumanTRANCE/TNFSF11/RANKL
Gln73-Asp317(Ala194Gly)
Accession#O14788 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot0.1µg/mLRecombinantHumanTRANCE/TNFSF11/RANK L(Truncated)(Catalog#390-TN)
- NeutralizationMeasuredbyitsABIlitytoneutralizeTRANCE/TNFSF11/RANK L-inducedosteoclast-likecellformationinRAW264.7mousemacrophages.TheNeutralizationDose(ND50)istypically0.01-0.04 µg/mLinthepresenceof 10 ng/mLRecombinantHumanTRANCE/TNFSF11/RANK L.
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Wong,B.R.et al.(1997)J.Biol.Chem.272:25190.
- Anderson,D.M.etal.(1997)Nature390:175.
- Nakagawa,N.etal.(1998)Biochem.Biophys.Res.Commun.245:382.
- Kong,Y-Y.etal.(1999)Nature397:315.
- LongName:TNF-relatedActivation-inducedCytokine
- EntrezGeneIDs:8600(Human);21943(Mouse);117516(Rat)
- AlternateNames:CD254antigen;CD254;ODF;OPGL;OPGLOPTB2;Osteoclastdifferentiationfactor;Osteoprotegerinligand;RANKL;RANKL;RANKLreceptoractivatorofnuclearfactorkappaBligand;Receptoractivatorofnuclearfactorkappa-Bligand;sOdf;TNF-relatedactivation-inducedcytokine;TNFSF11;TRANCE;TRANCEODFhRANKL2;tumornecrosisfactor(ligand)superfamily,member11;tumornecrosisfactorligandsuperfamilymember11
Background:
RANKLigand(receptoractivatorofNF-kappaBligand[RANKL],alsocalledTNF-relatedactivation-inducedcytokines(TRANCE),osteoprotegerinligand[OPGL],andosteoclastdifferentiationfactor[ODF]),isamemberofthetumornecrosisfactor(TNF)family.RANKLigandwasoriginallyidentifiedasanimmediateearlygeneupregulatedbyTcellreceptorstimulation.ThehumanRANKLigandCDNAencodesatypeIItransmembraneproteinof317aminoacidswithapredictedcytoplasmicdomainof47 aminoacids,a21aminoacidstransmembraneregion,andanextracellulardomainof249aminoacids.TheextracellulardomaincontainstwopotentialN‑linkedglycosylationsites.MouseandhumanRANKLigandshare85%aminoacididentity.RANKLigandisprimarilyexpressedinTcellsandTcellrichorgans,suchasthymusandlymphnodes.Themulti-functionsofRANKLigandincludeinductionofactivationofthec-junN‑terminalkinase,enhancementofTcellgrowthanddendriticcellfunction,inductionofosteoclastogenesis,andlymphnodeorganogenesis.RANKisthecellsurfacesignalingreceptorofRANKLigand.RANKhasbeenshowntoundergoreceptorclusteringduringsignaltransduction.Osteoprotegerin,asolublememberoftheTNFreceptorfamilywhichbindsRANKLigand,isanaturallyoccurringdecoyreceptorthatcounterbalancestheeffectsofRANKLigand.
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