
- SpeciesReactivityMouse
- SpecificityDetectsmouseIFN-gammaR1/CD119 inELISAs.Insandwichimmunoassays,nocross-reactivityorinterference withrecombinantmouse(rm) IFN‑alpha/beta R1,rmIFN-gammaR2,recombinanthuman(rh) IFN-gammaR1,rhIL-20Ralpha,rhIL-20Rbeta,rmIL-20Ralpha,rhIL-22BPorrmIL-22BPisobserved.
- SourceMonoclonalRatIgG2BClone#170911
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseIFN-gamma R1/CD119
Gly23-Asp253(predicted)
Accession#NP_034641 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- MouseIFN-gammaR1SandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLMouseIFN‑gamma R1/CD119Antibody(Catalog#MAB10262)
- ELISADetection(MatchedAntibodyPair)0.1-0.4µg/mLMouseIFN‑gamma R1/CD119BiotinylatedAntibody(Catalog#BAF1026)
- ELISAStandardRecombinantMouseIFN-gammaR1FcChimeraProtein,CF(Catalog#1026-GR)
- NeutralizationMeasuredbyitsABIlitytoneutralizeIFN‑gamma R1/CD119-mediatedinhibitionofEMCV-inducedcytopathyintheL‑929mousefibroblastcellline.Vogel,S.N.etal.(1982)Infect.Immun.38:681.TheNeutralizationDose(ND50)istypically4-20 ng/mLinthepresenceof1 ng/mLRecombinantMouseIFN‑gamma.
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Bach, E.A.et al.(1997)Annu.Rev.Immunol.15:563.
- LongName:InterferongammaReceptor1
- EntrezGeneIDs:3459(Human);15979(Mouse)
- AlternateNames:AVP,type2;CD119antigen;CD119;FLJ45734;IFN-gammaR1;IFN-gammareceptor1;IFNgammaR1;IFN-gamma-R1;IFNGR;IFNGR1;IFN-gR1;immuneinterferonreceptor1;interferongammareceptor1;interferon-gammareceptoralphachain;type2
Background:
Thehigh-affinityIFN-gamma receptorcomplexismadeupoftwotype Imembraneproteins,IFN-gamma R1(IFN-gamma Ralpha)andIFN-gamma R2(IFN-gamma Rbeta).Bothproteinsaremembersofthetype IIcytokinereceptorfamilyandshareapproximately52%overallsequenceidentity.IFN-gamma R1istheligand-bindingsubunitthatisnecessaryandsufficientforIFN-gammabindingandreceptorinternalization.IFN-gamma R2isrequiredforIFN-gammasignalingbutdoesnotbindIFN-gammabyitself.HumanIFN-gamma R1CDNAencodesa499 aminoacid(aa)proteinwitha17 aasignalpeptide,a228 aaextracellulardomain,a23 aatransmembranedomain,anda221 aaintracellulardomain.HumanandmouseIFN-gamma R1share52%aminoacidsequencesimilarityandbindIFN-gammainaspecies-specificmanner.IFN-gamma R1isconstitutivelyexpressedinmostcelltypes.SolubleIFN-gamma R1thatbindsIFN-gammahasbeendetectedinBIOLOGicalfluids.
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