- Species ReactivityMouse
- SpecificityDetects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL‑22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.
- SourceMonoclonal Rat IgG2A Clone # 496514
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1
Thr18-Ala228
Accession # Q80XZ4 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelPerCP (Peridinin-chlorophyll Protein Complex)
- Flow Cytometry10 µL/106 cellsSee below
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
- Tachiiri, A. et al. (2003) Genes Immun. 4:153.
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- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
- Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
- Wolk, K. et al. (2004) Immunity 21:241.
- Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
- Long Name:Interleukin 22 Receptor
- Entrez Gene IDs:58985 (Human); 230828 (Mouse)
- Alternate Names:CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
Background:
The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). Mouse IL-22 R alpha 1 contains a 215 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic region (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%‑26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R betaor IL-20 R betato form receptor complexes with distinct ligand selectivities. IL-10 R betais a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R betais a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R betais an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R betais an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL‑22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R betaor IL-20 R betaserves to stabilize the complex (3, 6‑9). IL‑22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R betaor IL-20 R betais required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL‑22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11‑14). IL‑22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL‑22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).
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