
- Species ReactivityHuman
- SpecificityDetects human FCRL2/FcRH2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FCRL1, 3, 4, or 5 is observed.
- SourceMonoclonal Mouse IgG1 Clone # 296902
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2
Glu15-Asp395
Accession # Q96LA5 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot1 µg/mLRecombinant Human FCRL2/FcRH2 (Catalog # 2048-FC)
- Flow Cytometry2.5 µg/106 cellsSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
- Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
- Miller, I. et al. (2002) Blood 99:2662.
- Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
- Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
- Hatzivassiliou, G. et al. (2001) Immunity 14:277.
- Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Huttmann, A. et al. (2006) Leukemia 20:1774.
- Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
- Li, F.J. et al. (2008) Blood 112:179.
- Long Name:Fc Receptor-like 2
- Entrez Gene IDs:79368 (Human)
- Alternate Names:CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FCRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4
Background:
Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3‑5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>


暂无品牌分类