- SpeciesReactivityMouse
- SpecificityDetectsmouseM-CSFR/CD115indirectELISAs.IndirectELISAs, nocross-reactivitywithrecombinanthumanM-CSFR/CD115,recombinantmouse(rm) PDGF Rbeta,orrmFlt-3Ligandisobserved.
- SourceMonoclonalRatIgG2BClone#460615
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouseM-CSFR/CD115
Ala20-Ser511
Accession#P09581 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- FlowCytometry0.25µg/106cellsSeebelow
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- deParseval,N.etal.(1993)NucleicAcidsRes.21:750.
- Rothwell,V.M.andL.R.Rohrschneider(1987)OncogeneRes.1:311.
- Chitu,V.andE.R.Stanley(2006)Curr.Opin.Immunol.18:39.
- Ross,F.P.andS.L.Teitelbaum(2005)Immunol.Rev.208:88.
- Rovida,E.etal.(2001)J.Immunol.166:1583.
- Yeung,Y.etal.(1998)J.Biol.Chem.273:17128.
- Dai,X.etal.(2002)Blood99:111.
- Faccio,R.etal.(2003)J.Clin.Invest.111:749.
- Li,M.etal.(2004)J.NeuRochem.91:623.
- Mitrasinovic,O.M.etal.(2005)J.Neurosci.25:4442.
- LongName:MacrophageColonyStimulatingFactorReceptor
- EntrezGeneIDs:1436(Human)
- AlternateNames:CD115antigen;CD115;c-fms;colonystimulatingfactor1receptor;CSF1R;CSF-1-R;CSFR;EC2.7.10.1;FMSproto-oncogene;FMSFIM2;macrophagecolonystimulatingfactorIreceptor;macrophagecolony-stimulatingfactor1receptor;McDonoughfelinesarcomaviral(v-fms)oncogenehomolog;M-CSFR;MCSFR;M-CSFR;Proto-oncogenec-Fms
Background:
M-CSFreceptor,theproductofthec-fmsproto-oncogene,isamemberofthetype IIIsubfamilyofreceptortyrosinekinasesthatalsoincludesreceptorsforSCFandPDGF.Thesereceptorseachcontainfiveimmunoglobulin-likedomainsintheirextracellulardomain(ECD)andasplitkinasedomainintheirintracellularregion(1-4).M-CSFreceptorisexpressedprimarilyoncellsofthemonocyte/macrophagelineage,dendriticcells,stemcellsandinthedevelopingplacenta(1).MouseM-CSFreceptorCDNAencodesa977aminoacid(aa)type Imembraneproteinwitha19 aasignalpeptide,a492 aaextracellularregioncontainingtheligand-bindingdomain,a25aatransmembranedomainanda441 aacytoplasmicdomain.ThemouseM-CSF RECDshares>99%aaidentitywithratand60-63%aa identitywithcorrespondingsequencesinhuman,canine,felineandbovineM-CSF R.ActivatorsofproteinkinaseCinduceTACE/ADAM17cleavageoftheM-CSFreceptor,releasingthefunctionalligand-bindingextracellulardomain(5).M-CSFbindinginducesreceptorhomodimerization,resultingintransphosphorylationofspecificcytoplasmictyrosineresiduesandsignaltransduction(6).TheintracellulardomainofactivatedM-CSFRbindsmorethan150 proteinsthataffectcellproliferation,survival,differentiationandcytoskeletalreorganization.Amongthese,PI3Kinase,P42/44ERKandc-CblarekeytransducersofM-CSFRsignals(3, 4).M-CSFRengagementiscontinuouslyrequiredformacrophagesurvivalandregulateslineagedecisionsandmaturationofmonocytes,macrophages,osteoclastsandDC(3, 4).M-CSFRandintegrinalphavbeta3sharesignalingpathwaysduringosteoclastogenesis,anddeletionofeithercausesosteopetrosis(7, 8).Inthebrain,microgliaexpressingincreasedM-CSFRareconcentratedwithAlzheimersabetapeptide,buttheirroleinpathogenesisisunclear(9, 10).
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
暂无品牌分类
