
- SpeciesReactivityHuman
- SpecificityDetectshumanTRAILR3/TNFRSF10Cin ELISAsandWesternblots.InWesternblots,nocross-reactivitywithrecombinanthuman(rh) TRAIL R1,rhTRAIL R2,orrhTRAIL R4isobserved.
- SourceMonoclonalMouseIgG1Clone#90905
- PurificationProteinAorGpurifiedfromascites
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanTRAILR3/TNFRSF10C
Ala26-Ala221
Accession#O14798 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLRecombinantHumanTRAIL R3/TNFRSF10CFcChimera(Catalog#630-TR)
- Immunohistochemistry8-25µg/mLSeebelow
- HumanTRAILR3/TNFRSF10CSandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLHumanTRAIL R3/TNFRSF10CAntibody(Catalog#MAB6301)
- ELISADetection(MatchedAntibodyPair)0.1-0.4µg/mLHumanTRAIL R3/TNFRSF10CBiotinylatedAntibody(Catalog#BAF630)
- ELISAStandardRecombinantHumanTRAILR3/TNFRSF10CFcChimeraProtein(Catalog#630-TR)
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Sheridan, J.P.et al.(1997)Science277:818.
- Golstein,P.(1997)Curr.Biol.7:R750.
- LongName:TRAILReceptor3
- EntrezGeneIDs:8794(Human)
- AlternateNames:AntagoNISTdecoyreceptorforTRAIL/Apo-2L;CD263antigen;CD263;cytotoxicTRAILreceptor-3;DcR1;DCR1MGC149502;Decoyreceptor1;DecoyTRAILreceptorwithoutdeathdomain;LITMGC149501;LymphocyteinhibitorofTRAIL;TNFRSF10C;TRAILR3;TRAILreceptor3;TRAILreceptorwithoutanintracellulardomain;TRAILR3;TRAIL-R3;TRAILR3TNF-relatedapoptosis-inducingligandreceptor3;TRIDDCR1-TNFR;tumornecrosisfactorreceptorsuperfamilymember10C;tumornecrosisfactorreceptorsuperfamily,member10c,decoywithoutanintracellulardomain
Background:
HumanTRAILR3,alsocalledDcR1(decoyreceptor1),LIT,andTRIDanddesignatedTNFRSF10C,isaglycosyl-phosphatidylinositol-linkedmembraneproteinwhichbindsTRAIL(Apo2Ligand)withhighaffinity.TRAILR3hastheTRAIL-bindingextracellularcysteine-richdomainsbutlackstheintracellularsignallingdomain.Asaresult,bindingofTRAILtoTRAILR3doesnottransduceanapoptosissignal.ExpressionofTRAILR3hasbeenshowntoprotectcellsbearingTRAILR1and/orTRAIL R2fromTRAIL-inducedapoptosis.AsecondTRAILdecoyreceptor,whichbindsTRAILwithhigh-affinitybutantagonizesTRAIL-inducedapoptosis,namedTRAILR4,DcR2orTRUNDD,hasalsobeenreported.ThehumansolubleTRAILR3/FcchimeraneutralizestheabilityofTRAILtoinduceapoptosis.
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