- SpeciesReactivityHuman
- SpecificityDetectshumanSR-BIindirectELISAsandWesternblots.
- SourceMonoclonalMouseIgG1Clone#947007
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenHumanembryonickidneycelllineHEK293-derivedhumanSR-BI
- FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot2µg/mLSeebelow
- Immunohistochemistry5-25µg/mLSeebelow
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Calvo,D.andM.A.Vega(1993)J.Biol.Chem.268:18929.
- Swiss-ProtaccessionQ8WTV0
- Chadwick,A.C.andD.Sahoo(2013)Curr.Opin.Endocrinol.DiabetesObes.20:124.
- Hoekstra,M.etal.(2012)Curr.Opin.Lipidol.23:127.
- Vergeer,M.etal.(2011)N.Engl.J.Med.364:136.
- Guo,L.etal.(2011)J.LipidRes.52:2272.
- Saddar,S.etal.(2012)Circ.Res.112:140.
- Vishnyakova,T.G.etal.(2006)Proc.Natl.Acad.Sci.USA103:16888.
- Baranova,I.N.etal.(2012)J.Immunol.188:1371.
- Leelahavanichkul,A.etal.(2012)J.Immunol.188:2749.
- Guo,L.etal.(2009)J.Biol.Chem.284:19826.
- LongName:ScavengerReceptorClassB,MemberI
- EntrezGeneIDs:949(Human);20778(Mouse);25073(Rat)
- AlternateNames:CD36andLIMPIIanalogous1;CD36antigen(collagentypeIreceptor,thrombospondinreceptor)-like1;CD36antigen;CD36L1;CD36L1scavengerreceptorclassBtype1;CLA1CD36antigen-like1;CLA-1HDLQTL6;CLA1;CollagentypeIreceptor,thrombospondinreceptor-like1;HDLQTL6;MGC138242;SCARB1;scavengerreceptorclassBtypeIII;scavengerreceptorclassB,member1;SRB1scavengerreceptorclassBmember1;SR-B1;SRBI;SR-BI
Background:
ScavengerReceptor,classB,member1(SR-B1),genenameSCARB1,isalsoknownasCD36L1(CD36-like1)orCLA-1(CD36andLIMPIIanalogous1)(1-5).SR-B1isatransmembraneglycoproteinfoundonmacrophages,livercellsandothersteroidogeniccellsasalipoproteinreceptor.The552aminoacid(aa)humanSR-B1containsacentralextracellulardomain(ECD),flankedbyN-andC-terminaltransmembranedomains.HumansplicevariantsdifferattheN-terminalcytoplasmicandtransmembranedomains(SR-BIII,474aa),theN-terminalendoftheECD(SR-BII,409aa),ortheC-terminalcytoplasmicdomain(isoform3,552aa)(2).ThehumanSR-B1ECDshares80%,80%,89%,86%and84%aasequenceidentitywithmouse,rat,porcine,rabbit,andbovineSR-B1,respectively.SR-B1functionsinreversecholesteroltransport(RCT),whichisthoughttobeanti-atherogenicbyfacilitatingtransportofcholesterylestersfrommacrophagesbacktotheliverfordegradation(3).Inrodenthepatocytes,SR-B1isthemainreceptormediatingRCT,whilehumanhepatocytesalsoexpressasecondmediator,CETP(cholesterylestertransferprotein)(3-5).TheimportanceofSR-B1inhumansisshownbyhumanSR-B1geneticvariantsthatalterlipidmetabolism(3-7).Forexample,theP297Spolymorphismlowersuptakeofhigh-densitylipoprotein(HDL)cholesterolintheliverandincreasesplasmaHDLcholesterol(3-5).Onendothelialcells,signalingthroughSR-B1activatesnitricoxideproduction,whichattenuatesmonocyteadhesion(6).Onadrenocorticalcells,SR-B1mediatesuptakeofcholesterylestersfromHDLforthesynthesisofglucocorticoidhormonessuchascortisol(3-5).Onplatelets,HDLbindingtosurfaceSR-B1inhibitsaggregationandincreasesplateletsurvivaltime(3-5).Onhumanovariangranulosacells,deficiencyofSR-B1correlateswithlowfertility(3).SR-B1anditsSR-BIIisoformalsobindbacteriallipopolysaccharides,facilitatinguptakeofvariousbacteriabycellssuchasperitonealmacrophages(8,9).Thisuptakeenhancesinflammatoryresponseswhich,unlessproperlycontrolled,canresultinsepsis(9-11).
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
暂无品牌分类
