
- SpeciesReactivityMouse
- SpecificityDetectsmouse4-1BBindirectELISAsandWesternblots.Doesnotcross-reactwithrecombinanthuman(rh)4‑1BB,rhCD27,recombinantmouse(rm)CD27,rhCD30,rmCD30,rhCD40,rmCD40,rhDR3,rhDR6,rhEDAR,rmEDAR,rhFas,rmFas,rhGITR,rmGITR,rhHVEM,rhLTRbeta,rhNGF R,rhOPG,rmOPG,rhRANK,rmRANK,rhTROY,rmTROY,rhTNF sRI,rhTNF sRII,orrmTNF sRII.
- SourceMonoclonalRatIgG2AClone#158332
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinantmouse4-1BB
Val24-Leu187
Accession#P20334 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot1µg/mLRecombinantMouse4-1BB/TNFRSF9/CD137FcChimera(Catalog#937-4B)
undernon-reducingconditionsonly - FlowCytometry0.25µg/106cellsSeebelow
- CyTOF-readyReadytobelabeledusingestablishedconjugationmethods.NoBSAorothercarrierproteinsthatcouldinterferewithconjugation.
- Mouse4-1BB/TNFRSF9/CD137SandwichImmunoassayReagent
- ELISACapture(MatchedAntibodyPair)2-8µg/mLMouse4‑1BB/TNFRSF9/CD137Antibody(Catalog#MAB937)
- ELISADetection(MatchedAntibodyPair)0.1-0.4µg/mLMouse4‑1BB/TNFRSF9/CD137BiotinylatedAntibody(Catalog#BAF937)
- ELISAStandardRecombinantMouse4-1BB/TNFRSF9FcChimeraProtein,CF(Catalog#937-4B)
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Goodwin,R.G.etal.(1993)Eur.J.Immunol.23:2631.
- Alderson,M.R.etal.(1994)Eur.J.Immunol.24:2219.
- Kwon,B.S.andS.M.Weissman(1989)Proc.Nat.Acad.Sci.USA86:1963.
- Wilcox,R.A.etal.(2002)J.Immunol.168:4262.
- Kwon,B.etal.(2002)TRENDSinImmunology23:378.
- EntrezGeneIDs:3604(Human);21942(Mouse);500590(Rat);102127961(CynomolgusMonkey)
- AlternateNames:4-1BBligandreceptor;41BB;4-1BB;CD137antigen;CD137;CD137MGC2172;CDw137;FLJ43501;ILA;ILAhomologofmouse4-1BB;inducedbylymphocyteactivation(ILA);interleukin-activatedreceptor,homologofmouseLy63;receptorprotein4-1BB;TcellantigenILA;T-cellantigen4-1BBhomolog;T-cellantigenILA;TNFRSF9;tumornecrosisfactorreceptorsuperfamilymember9;tumornecrosisfactorreceptorsuperfamily,member9
Background:
4-1BB,alsoknownasCD137andILA(inducedbylymphocyteactivation),isaTNFreceptorsuperfamilymemberandhasbeendesignatedTNFRSF9.Mouse4-1BBCDNAencodesa256aminoacid (aa)residuestypeItransmembraneproteinwithaputative23aasignalpeptide,a164aaextracellulardomain,a21aatransmembranedomainanda48aacytoplasmicregion(1-3).Asoluble4-1BBisreleasedfromsurfacesofcellsexpressingthetransmembraneprotein(4).Mouse4‑1BBsharesapproximately60%aasequenceidentitywithitshumancounterpart.4-1BBisexpressedonactivatedCD4+andCD8+Tcells,thymocytes,andNKcells.Itisalsoexpressedonmonocytes,neutrophils,DCsandeosinophils(5).Theligandfor4-1BB(4-1BBL),alsonamedTNFSF9,belongstotheTNFligandsuperfamily.4-1BBLispredominantlyexpressedonactivatedantigenpresentingcells(APCs)suchasBcells,macrophagesanddendriticcells(DCs).ItisalsoexpressedonmostTandBlymphomacelllines.Inresponseto4-1BBLbinding,4-1BBtransduceaTcellcostimulatorysignalinbothCD4+andCD8+Tcellstopromotesurvivalandenhanceproliferation,cytokineproductionandeffectorfunction.Invivo,thecostimulatoryactivityof4-1BBhasbeenshowntobeimportantingraft-vs-hostdiseaseandantiviralCTLresponses.Ondendriticcells,4-1BBisaDC-activatingmoleculesthatenhancescytokineproductionandupregulatesexpressionofB7-1andB7‑2costimulatorymolecules,resultinginanimprovedabilitytostimulateTcellresponses(1-5).
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