- SpeciesReactivityHuman
- SpecificityDetectshumanIntegrinbeta2/CD18indirectELISAsandWesternblots.IndirectELISAs,nocross-reactivitywithrecombinanthuman Integrin beta1orrecombinantmouseIntegrin alpha5isobserved.
- SourceMonoclonalMouseIgG1Clone#212701
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanIntegrin beta2/CD18
Gln23-Asn700
Accession#AAA59490 - FormulationSuppliedinasalinesolutioncontainingBSAandSodiumAzide.
- LabelFluorescein
- FlowCytometry10µL/106cellsSeebelow
- ShippingTheproductisshippedwithpolarpacks.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.
- StABIlity&StorageProtectfromlight.Donotfreeze.
- 12monthsfromdateofreceipt,2to8°Cassupplied.
- Corbi,A.L.etal.(1987)EMBOJ.6:4023.
- Kishimoto,T.K.etal.(1987)Cell48:681.
- Hynes,R.O.(2002)Cell110:673.
- Arnaout,M.A.(1990)Blood75:1037.
- Postigo,A.A.etal.(1991)J.Exp.Med.174:1313.
- Beyer,M.etal.(2005)Respir.Res.6:70.
- Nicolaou,F.etal.(2003)Blood101:4033.
- Vorup-Jensen,T.etal.(2003)Proc.Natl.Acad.Sci.USA100:1873.
- Bilsland,C.A.G.etal.(1994)J.Immunol.152:4582.
- Pendu,R.etal.(2006)Blood108:3746.
- Sadhu,C.etal.(2007)J.Leukoc.Biol.81:1395.
- Schack,L.etal.(2009)J.Immunol.182:6943.
- Choi,J.etal.(2005)Biochem.Biophys.Res.Commun.331:557.
- Gang,J.etal.(2007)Mol.Cells24:240.
- Vorup-Jensen,T.etal.(2007)J.Biol.Chem.282:30869.
- Vorup-Jensen,T.etal.(2004)Proc.Natl.Acad.Sci.USA102:1614.
- Kishimoto,T.K.etal.(1987)Cell50:193.
- EntrezGeneIDs:3689(Human);16414(Mouse);309684(Rat)
- AlternateNames:959betasubunitprecursor)10;CD18antigen;CD18;CD18beta2;cellsurfaceadhesionglycoprotein(LFA-1;CR3;Integrinbeta2;integrinbeta-2;integrin,beta2(antigenCD18(p95),lymphocytefunction-associatedantigen1;macrophageantigen1(mac-1)betasubunit);integrin,beta2(complementcomponent3receptor3and4subunit);ITGB2;LAD;LCAMB;leukocytecelladhesionmoleculeCD18;leukocyte-associatedantigensCD18/11A,CD18/11B,CD18/11C;LFA-1beta;LFA-1;MAC-1beta;MF17;MFI7;P150;p150,95beta
Background:
IntegrinalphaXbeta2,alsocalledCD11c/CD18,p150/95orcomplementreceptortype4(CR4),isoneoffourbeta2integrins.Thenon-covalentheterodimerof150kDaalphaX/CD11cand95kDabeta2/CD18integrinsubunitsisexpressedonmacrophages,dendriticcellsandhairycellleukemias,withloweramountsonothermyeloidcellsandactivatedB,NKandsomecytotoxicT cells(1‑7).Likeotherintegrins,alphaXbeta2hasmultipleactivationstates(3).Inthepresenceofdivalentcationsand"inside-out"signaling,alphaXbeta2isfullyactiveandextended.ThealphaXvWFAorI-domain,whichcontainstheadhesionsites,formstheN-terminalheadregionwiththealphaXbeta-propellerandthebeta2vWFAdomain(1,8).Intheinactivestate,theheterodimerflexesinthecenteratthealphaXthighandcalfdomainsandbeta2I-EGFdomains,impedingaccesstoadhesionsites(1).The1088aahumanalphaX/CD11cECDshares70‑76%aasequenceidentitywithmouse,ratandcaninealphaXwhilethe678 aahumanbeta2/CD18ECDshares81‑83%aasequenceidentitywithmouse,rat,cow,dog,goat,sheep,andpigbeta2.PotentialalphaXisoformscontaining719and725aa(ascomparedtofull-length1163aaalphaX)lackthevWFAdomainandtheN-terminus.ActivealphaXbeta2sharessomeadhesionpartnerswithalphaMbeta2/CD11b/CD18,includingcomplementopsoninfragmentiC3b,ICAMs,vWFandfibrinogen,andisexpressedonmanyofthesamecells(4‑11).However,alphaMbeta2activityisoftenconstitutive,whilealphaXbeta2activityrequirescellactivation(4‑7).alphaXbeta2alsobindsosteopontin,Thy-1,plasminogen,heparin,andproteinswithabnormallyexposedacidicresidues(11‑16).Theadhesioneventsareimportantforproliferation,degranulation,chemotacticmigration,andphagocytosisofcomplement-opsonizedparticles(5,6,9,11,12,16).Mutationsofbeta2,especiallyinthevWFAdomain,causeleukocyteadhesiondeficiency(LAD-1)andsusceptibilitytobacterialinfections(17).
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