
- Species ReactivityHuman, Mouse
- SpecificityDetects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA2 and rhCA3 is observed and less than 1% cross-reactivity with rhCA1, rhCA4, and rhCA9 is observed.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot0.5 µg/mLSee below
- Simple Western5 µg/mLSee below
- Immunoprecipitation25 µg/mLConditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VIII (Catalog # 2187-CA), see our available Western blot detection antibodies
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
- Hirota, J. et al. (2003) Biochem. J. 372: 435.
- Miyaji, E. et al. (2003) J. Pathol. 201: 37.
- Lu, S.H. et al. (2004) Lung Cancer 44: 273.
- Entrez Gene IDs:767 (Human)
- Alternate Names:CA8; CALS; CALScarbonate dehydratase; CAMRQ3; Carbonic Anhydrase VIII; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502
Background:
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA8, also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site (2). Nevertheless, it has esterase activity described in the Activity Assay Protocol. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5‑triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5).
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