
- SpeciesReactivityHuman,Mouse
- SpecificityDetectsrecombinant mouse RNF168andrecombinanthumanRNF168 indirectELISAsandWesternblots.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenE.coli-derivedrecombinantmouseRNF168
Asn423-Arg565
Accession#Q80XJ2 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot0.5µg/mLSeebelow
- ImmunocytochemistryThisantibodyhasbeenusedataconcentrationof5-15μg/mLtodetectRNF168inimmersionfixedHeLahumancervicalepithelialcarcinomacellline. Internaltestingwasnotabletovalidatestaininginamousecellline.
- ReconstitutionSterilePBStoafinalconcentrationof0.2mg/mL.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:RingFingerProtein168
- EntrezGeneIDs:165918(Human);70238(Mouse)
- AlternateNames:E3ubiquitin-proteinligaseRNF168;EC6.3.2;EC6.3.2.-;FLJ39749;ringfingerprotein168FLJ35794;RNF168
Background:
RNF168(RING[reallyinterestingnewgene]fingerprotein168;alsoE3ubiquitin-proteinligaseRNF168)isa 65kDa(predicted) memberoftheRNF168familyofproteins.Itisubiquitouslyexpressed,andservesasanE3ubiquitinligase.FollowingDNAdamage,RNF8isrecruitedtoDNAdouble-strandbreaksbyphosphoMDC1.Here,RNF8firstmonoubiquitinateshistoneH2A,andthenpromotesRNF168recruitment.RNF168nowactsasanadditionalubiquitinase,promotingmultipleubiquitinationsplustherecruitmentof53BP1,ascaffoldproteinthatholdsDNAdamageresponseelements.MouseRNF168is565aminoacids(aa)inlength.ItcontainsoneZn-finger/RINGdomain(aa16-55)plustwoubiquitin-interactingMIUmotifs(aa168-191and438-461).TherearethreepotentialSer/Thrphosphorylationsites.Twopotentialisoformvariantsarereported.OneshowsanalternativestartsiteatMet20,whileasecondpossessesatwoaaextensionattheN-terminuscoupledtoa13aasubstitutionforaa343-565.Overaa423-565,mouseRNF168shares79%and65.5%aasequenceidentitywithratandhumanRNF168,respectively.Overaa423-466,mouseRNF168shares98%aasequenceidentitywithhumanRNF168.
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