
- Species ReactivityHuman, Mouse
- SpecificityDetects recombinant mouse RNF168 and recombinant human RNF168 in direct ELISAs and Western blots.
- SourcePolyclonal Sheep IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant mouse RNF168
Asn423-Arg565
Accession # Q80XJ2 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot0.5 µg/mLSee below
- ImmunocytochemistryThis antibody has been used at a concentration of 5-15μg/mL to detect RNF168 in immersion fixed HeLa human cervical epithelial carcinoma cell line. Internal testing was not able to validate staining in a mouse cell line.
- ReconstitutionSterile PBS to a final concentration of 0.2 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Ring Finger Protein 168
- Entrez Gene IDs:165918 (Human); 70238 (Mouse)
- Alternate Names:E3 ubiquitin-protein ligase RNF168; EC 6.3.2; EC 6.3.2.-; FLJ39749; ring finger protein 168FLJ35794; RNF168
Background:
RNF168 (RING [really interesting new gene] finger protein 168; also E3 ubiquitin-protein ligase RNF168) is a 65 kDa (predicted) member of the RNF168 family of proteins. It is ubiquitously expressed, and serves as an E3 ubiquitin ligase. Following DNA damage, RNF8 is recruited to DNA double-strand breaks by phosphoMDC1. Here, RNF8 first monoubiquitinates histone H2A, and then promotes RNF168 recruitment. RNF168 now acts as an additional ubiquitinase, promoting multiple ubiquitinations plus the recruitment of 53BP1, a scaffold protein that holds DNA damage response elements. Mouse RNF168 is 565 amino acids (aa) in length. It contains one Zn-finger/RING domain (aa 16-55) plus two ubiquitin-interacting MIU motifs (aa 168-191 and 438-461). There are three potential Ser/Thr phosphorylation sites. Two potential isoform variants are reported. One shows an alternative start site at Met20, while a second possesses a two aa extension at the N-terminus coupled to a 13 aa substitution for aa 343-565. Over aa 423-565, mouse RNF168 shares 79% and 65.5% aa sequence identity with rat and human RNF168, respectively. Over aa 423-466, mouse RNF168 shares 98% aa sequence identity with human RNF168.
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