- SpeciesReactivityHuman,Mouse
- SpecificityDetectshumanandmouseCaspase-2precursorandthesmallCaspase-2subunitthatisgeneratedduringproteolyticactivationinWestern blots.
- SourcePolyclonalRabbitIgG
- PurificationAntigenAffinity-purified
- ImmunogenKLH-coupledmouseCaspase-2syntheticpeptide
KEREGYAPGTEFHRC - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot0.8µg/mLSeebelow
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- Chowdhury,I.etal.(2008)Comp.Biochem.Physiol.B151:10.
- Krumschnabel,G.etal.(2009)CellDeathDiffer.16:195.
- Kitevska,T.etal.(2009)Apoptosis14:829.
- Paroni,G.etal.(2001)J.Biol.Chem.276:21907.
- Li,H.etal.(1997)J.Biol.Chem.272:21010.
- SwissProt.Accession #P42575.
- Wang,L.etal.(1994)Cell78:739.
- Chang,D.W.etal.(2003)J.Biol.Chem.278:16466.
- Olsson,M.etal.(2009)Oncogene28:1949.
- Tinel,A.&J.Tschopp(2004)Science304:843.
- Schweizer,A.etal.(2003)J.Biol.Chem.278:42441.
- Colussi,P.A.etal.(1998)J.Biol.Chem.273:24535.
- Droin,N.etal.(2000)CancerRes.60:7039.
- EntrezGeneIDs:835(Human);12366(Mouse)
- AlternateNames:caspase-2;CASP2;CASP-2;caspase2,apoptosis-relatedcysteinepeptidase;Caspase2;Caspase-2;ICH1;ICH-1;NEDD2;NEDD-2;PPP1R57
Background:
Caspase-2(Cysteine-asparticacidprotease2/Casp2;alsoNEDD2andICH-1)isa30‑32kDamemberofthepeptidaseC14A/IL-1beta-convertingfamilyofenzymes(1‑3).Itiswidelyexpressedandisanintegralcomponentoftheapoptoticcascade.Basedonthelengthofitsprodomain,Caspase‑2hasbeenconsideredtobeaninitiatorcaspase.However,studieshaveshownthatothercaspases(suchasCasp3)activateprocaspase2,andCaspase‑2likelyactsonkeycellularmolecules suchasBID,Golgin160andDFF45/ICAD(2,4,5).Thus,Caspase‑2isperhapsmorelikelytobeaspecializedexecutionercaspase.Humanprocaspase‑2isa48‑51 kDa,452aminoacid(aa)protein(4‑7).Itisknowntoexistasadisulfide‑linkedhomodimerviacovalentlinkageatCys436(2,5).Butthisdimericstatemaynotbesufficientfor(auto)activation.ActualactivationmayoccurfollowingoligomerizationwithinthecontextofactivatingplatformssuchasDISC(death‑inducingsignaling complex)orthePIDDosome(8‑10).Initially,procaspase‑2undergoesproteolyticcleavagetogenerateanN‑terminal333aap34/34kDasubunit,anda119 aaC‑terminalp14/14kDasubunit(5).Thep34andp14subunitsarefurtherprocessedtogeneratetheprodomain(aa1‑169),plusthematurep18(aa170‑333)andp12(aa348‑452)subunits(4‑6).Notably,eachp18:p12noncovalentheterodimerdemonstratesproteolyticactivityaroundacatalyticsiteataa318‑322,and,duetoannuclearlocalizationsignalwithintheprodomain,maybefoundineithernucleusorcytoplasm(11,12).Therearemultiplepotentialisoformvariants.Individually,orincombination,thereisanalternativestartsiteatMet18,asubstitutionoftwoaaforaa 107‑452,asecondsubstitutionof14aaforaa309‑322,andathirdsubstitutionof22aaforaa323‑452(6,7,13).Thehumanandmouseprocaspase2precursorsare90%aaidentical,withthemajorityofdifferenceslyingintheprodomain.
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