
- SpeciesReactivityHuman,Mouse
- SpecificityDetectshumanandmouse PolypeptideGalNacTransferase10/GALNT10inWesternblots.IndirectELISA,lessthan1%cross-reactivitywithrecombinanthuman(rh)GALNT1andrhGALNT4isobserved.
- SourcePolyclonalSheepIgG
- PurificationAntigenAffinity-purified
- ImmunogenMousemyelomacelllineNS0-derivedrecombinanthumanPolypeptideGalNacTransferase10/GALNT10
Leu71-Asn603
Accession#Q86SR1 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot0.5µg/mLSeebelow
- Immunohistochemistry5-15µg/mLSeebelow
- ReconstitutionSterilePBStoafinalconcentrationof0.2mg/mL.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:UDP-GalNAc:polypeptideN-acetylgalactosaminyltransferase10
- EntrezGeneIDs:55568(Human);171212(Mouse);170501(Rat)
- AlternateNames:DKFZp586H0623;EC2.4.1.41;FLJ00205;FLJ11715;GalNActransferase10;GALNACT10;GalNAc-T10;GALNT10;PolypeptideGalNActransferase10;polypeptideN-acetylgalactosaminyltransferase10;PPGALNACT10;pp-GaNTase10;PPGANTASE10;Protein-UDPacetylgalactosaminyltransferase10;UDP-GalNAc:polypeptideN-acetylgalactosaminyltransferase10;UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase10(GalNAc-T10)
Background:
GALNT10(N-Acetyl-GalactosaminylTransferase10;alsoUDP-Acetylgalactosaminyltransferase10andppGalNAc-T10)isamemberoftheGalNACtransferasesubfamily,glycosyltransferase2familyofenzymes.Itiswidelyexpressed,beingfoundinintestine,pancreas,thyroidandspleen.GALNT10isfoundintheGolgiapparatus,andcatalyzesthetransferofUDP-GalNAcontoeitheraSerorThrresidueonapreviouslyglycosylatedpeptide/polypeptidebackbone.HumanGALNT2isa603aminoacid(aa)typeIItransmembraneglycoprotein.Itcontainsan11aaN-terminalcytoplasmicregionanda572aaextracellulardomain(aa32-603).TheECDpossessestwokeyparts,acatalyticregionwithtwocatalyticsuBDomains(aa144-253and311-373),andaricinB-typelectindomainthatbindscarbohydrates(aa458-590).Thesetwodistinctdomainshaveuniquebutcomplimentaryproperties.Byitself,anactivecatalyticdomaincandonomorethanplaceaGalNAcresidueimmediatelyadjacenttoanexistingC-terminalGalNACresidue.Alternatively,ifthelectindomainisinvolved,thisdomainmayrecognize(bindto)anynumberofexistingglycosylationsites,allowingforthesubsequentattachmentofGalNAcbythecatalyticsiteontoaSerorThrresiduequitedistantfromtheinitiallectindomain:CHOrecognitionsite.Thereareatleastthreepotentialspliceformvariants.Oneshowsadeletionofaa190-251,asecondcontainsa13aasubstitutionforaa354-366,andathirdpossessesa23aasubstitutionforaa1-352coupledtoathreeaasubstitutionforAsn389.Overaa71-603,humanGALNT10shares96%aasequenceidentitywithmouseGALNT10.
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