
- SpeciesReactivityHuman,Mouse
- SpecificityDetectshumanMIFindirect ELISAandWesternBlots.
- SourceMonoclonalMouseIgG1Clone#932612
- PurificationProteinAorGpurifiedfromhybridomaculturesupernatant
- ImmunogenE.coli-derivedrecombinanthumanMIF
Met1-Phe114
Accession#P14174 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- LabelUnconjugated
- WesternBlot2µg/mLSeebelow
- Immunohistochemistry8-25µg/mLSeebelow
- ReconstitutionReconstituteat0.5mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- StABIlity&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
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- Flieger,O.etal.(2003)FEBSLett.551:78.
- Philo,J.S.etal.(2004)Biophys.Chem.108:77.
- Sun,H-W.etal.(1996)ProteinEng.9:631.
- Stamps,S.L.et.al.(2000)Biochemistry39:9671.
- Nguyen,M.T.etal.(2003)J.Biol.Chem.278:33654.
- Sato,A.etal.(2003)Dev.Comp.Immunol.27:401.
- Bernhagen,J.etal.(1994)Biochemistry33:14144.
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- Meyer-Siegler,K.L.andP.L.Vera(2005)J.Urol.173:615.
- LongName:MacrophageMigrationInhibitoryFactor
- EntrezGeneIDs:4282(Human);17319(Mouse)
- AlternateNames:EC5.3.2.1;EC5.3.3.12;GIFmacrophagemigrationinhibitoryfactor;GLIF;Glycosylation-inhibitingfactor;L-dopachromeisomerase;L-dopachrometautomerase;macrophagemigrationinhibitoryfactor(glycosylation-inhibitingfactor);MIF;MMIF;Phenylpyruvatetautomerase
Background:
MIF(ormacrophagemigrationinhibitoryfactor)wasthefirstlymphokine/cytokinetoberecognizedinthepregenomicsera(1, 2).Regardless,itisoneoftheleastunderstoodofallinflammatorymediators(1, 3).HumanMIFisa12.5kDa,115aminoacid(aa)nonglycosylatedpolypeptidethatissynthesizedwithoutasignalsequence(4-7).SecretionoccursnonclassicallyviaanABCA1transporter(8).TheinitiatingMetisremoved,leavingProasthefirstaminoacid.Themoleculeconsistsoftwoalpha-helicesandsixbeta-strands,fourofwhichformabeta-sheet.Thetworemainingbeta-strandsinteractwithotherMIFmolecules,creatingatrimer(2, 9, 10).Structure-functionstudiessuggestMIFisbifunctionalwithsegregatedtopology.TheN-andC-terminimediateenzymeactivity(intheory).Phenylpyruvatetautomeraseactivity(enol-to-keto)hasbeendemonstratedandisdependentuponProatposition#1(11).Aminoacids50 - 65havealsobeensuggestedtocontainthiol-proteinoxidoreductaseactivity(12).MIFhasproinflammatorycytokineactivitycenteredaroundaa’s49-65.Onfibroblasts,MIFinduces,IL-1,IL-8andMMPexpression;onmacrophages,MIFstimulatesNOproductionandTNF-alphareleasefollowingIFN-gammaactivation(13,14).MIFapparentlyactsthroughCD74andCD44,likelyinsomeformoftrimericinteraction(15,16).HumanMIFisactiveonmousecells(14).HumanMIFis90%,94%,95%,and90%aaidenticaltomouse,bovine,porcineandratMIF,respectively.
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