
- Species ReactivityHuman, Mouse
- SpecificityDetects human MYF-5 in direct ELISAs.
- SourceMonoclonal Mouse IgG2A Clone # 593128
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- Immunocytochemistry8-25 µg/mLSee below
- Intracellular Staining by Flow Cytometry0.25 µg/106 cellsSee below
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Myogenic Regulatory Factor 5
- Entrez Gene IDs:4617 (Human); 17877 (Mouse); 299766 (Rat)
- Alternate Names:bHLHc2; bHLHc2Class C basic helix-loop-helix protein 2; MYF5; MYF-5; myogenic factor 5
Background:
MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.
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