
- Species ReactivityHuman, Mouse
- SpecificityDetects human TOP2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse TOP2B and no cross-reactivity with recombinant human TOP2A is observed.
- SourceMonoclonal Mouse IgG2B Clone # 681417
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human TOP2B
Phe1192-Asp1621
Accession # Q02880 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Western Blot1 µg/mLSee below
- Immunocytochemistry8-25 µg/mLSee below
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:DNA Topoisomerase II beta
- Entrez Gene IDs:7155 (Human); 21974 (Mouse)
- Alternate Names:antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen
Background:
TOP2B (DNA Topoisomerase II beta) is a 180-185 kDa member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/a-form being 170 kDa in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relives torsional stress created in DNA during transcription or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626 amino acids (aa) in length. It contains an ATPase domain (aa 101-201), an NES (aa 1034-1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa 1508-1611). There is one splice variant that shows a deletion of aa 24-28. Over aa 1187-1621, human TOP2B shares 91% aa identity with mouse TOP2B.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>


暂无品牌分类