
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 29777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 29777-AAV1 | Limited Stock Available, 3 units leftVirus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backboneAAV with CAG promoter(Search Vector Database)
- Backbone manufacturerScott Sternson
- Backbone sizew/o insert(bp)5451
- Vector typeMammalian Expression, AAV ; Adeno-associated virus
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Growth instructionsUse recombinase-free E. coli (Stbl3, XL-10, Sure2 et.al), grow at 30C
- Copy numberLow Copy
Gene/Insert
- Gene/Insert nameArchT-GFP
- Alt namearchaerhodopsin
- Alt namearchaerhodopsin TP009
- Alt nameArchT
- SpeciesH. strain TP009
- Insert Size (bp)744
- MutationN/A
- Tag/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteBamHI(not destroyed)
- 3′ cloning siteEcoRI(not destroyed)
- 5′ sequencing primergctctagagcctctgctaacc
- 3′ sequencing primergcagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
- Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 12 References
Depositor Comments
PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
Information for AAV1 (Catalog # 29777-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pAAV-CAG-ArchT-GFP (#29777). In addition to the viral particles, you will also receive purified pAAV-CAG-ArchT-GFP plasmid DNA.
CAG-driven ArchT-GFP expression for optogenetic inhibition.These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
1二甲苯中脱蜡5-10分钟。
2换用新鲜的二甲苯,再脱蜡5-10分钟。
3无水乙醇5分钟。
490%乙醇2分钟。
570%乙醇2分钟
6蒸馏水2分钟。
从烤箱出来需要冷却吗?
2、【仪器型号】:SX-500
3、【生产厂家】:日本TOMY
4、【检测适用范围】:高压蒸汽灭菌
5、【仪器使用优点、缺点】:必填项。其中缺点若觉得不便评论的大家可以不写,但若评价较为客观科学,我们还是鼓励的!我想仪器公司见到此帖也会根据我们的建议和评价来改进他们的工作!
6、【仪器外观描述或者图片】:见附件。
7、【说明书或者操作规范】:
对于传统的水平装入的高压蒸汽灭菌器,方形的提篮放入圆形的内腔,浪费了接近36%的内腔体积。Tomy产品的上部装入式设计充分地优化利用了内腔的空间,将要灭菌的物品直接放入内腔中,或将要灭菌的物品放入圆形提篮(标准附件)中再放入内腔中。顶部装入设计使样品的处理更加容易和样品处理量增大。顶部装入设计使高压蒸汽灭菌器可以安装在地面上,不需要支架和台面或节约了有限的台面。SX系列高压灭菌器顶部的操作仅需单手或单脚即可操作;打开顶盖只需用脚踩下位于底部的开关踏板,顶盖锁即打开,顶子即向上自动打开;关闭顶盖也非常轻松容易,只需将盖子压下即可。集成式设计,节约安装和使用空间。标准配备一个快速空冷系统,使高温高压后的温度能快速的降低到常温,极大地缩短了操作时间;同时该功能可用于较低温度的循环灭菌操作。可以采用定时钟设定定时启动,节约实验预备时间。操作面板上设置大型的指示灯通过颜色变化来指示设备状态。灭菌操作的选择和容易观察的实时状态显示。图形显示对灭菌过程的选择,有五种常用的操作模式供选择,并可通过召回方式设定。同时操作面板上的LCD闪烁显示整个灭菌过程的进行状态,图形和温度及时间显示,在灭菌操作完成后排气阀自动打开,速度可设置在6个级别内设定(对液体灭菌设为速度可变)
顶盖锁定功能,在灭菌操作过程中顶盖配备高水平的锁定装置,以避免可能事故发生。可变的温度设定,使设置100℃如同设置121℃一样容易,设置温度范围从45℃到135℃,分辨为1℃(加热模式可达104℃)。水位高度传感器安全装置的作用有效地防止了干加热的发生。灭菌过程中在温度和压力平衡时,排气的自动微调功能使腔内的条件达到最佳。
8、【仪器维护及保养知识】:无特殊。
9、【参考价格】:选填项。
10【使用后的评价】:容易操作,空间利用效率高。自动化程度高,安全高效。
QQ:1917490559空间:http://user.qzone.qq.com/1917490559
本实验室技术力量雄厚,具有先进的病理技术设备(全自动免疫组化染色机,全自动HE染色机、全自动脱水机、包埋机、石蜡切片机等)。主要的病理技术服务有:
1、组织脱水、石蜡包埋;切片
2、HE染色;
3、特殊染色(网状纤维染色、Masson三色染色、弹力纤维染色、胶原纤维染色、尼氏体染色、油红O染色、六胺银染色等);
4、免疫组织化学色;
5、冰冻切片;
6、免疫荧光染色;
7、电镜技术(待定)。

