
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 105548 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 105548-AAV1 | Virus (100 µL at titer ≥ 1×10¹3 vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV5 | 105548-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV8 | 105548-AAV8 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameTurboRFP
- PromoterCMV
Cloning Information
- Cloning methodUnknown
Resource Information
- Supplemental Documents
- pENN.AAV.CMV.TurboRFP.WPRE.RBG (P2177).gb
- Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs
- Industry Terms
- Not Available to Industry
Depositor Comments
Penn Vector Core number p2177
Information for AAV1 (Catalog # 105548-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 105548-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV8 (Catalog # 105548-AAV8)(Back to top)
Purpose
Ready-to-use AAV8 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
ebiomall.com






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1、在使用前应该仔细阅读说明书,按说明书上面的步骤操作,关闭水嘴后先加水到水位线,再接通电源。
2、设置数显温度,打开电源开关使水箱升温,盖上水箱盖,待水温升到所需温度值,打开循环电源开关,水箱内的水在循环均匀室内温度。
3、数显电热恒温水浴锅水位不能过高,以防止水溢出造成实验失误,
4、为延长数显电热恒温水浴锅使用寿命,请注意勿将控制箱内受潮,防止漏电。
5、恒温水浴锅自出厂之日起保修一年,终身维修
6、数显电热恒温水浴锅使用结束后,请将水从出水口放干净,用干布擦拭干净,置于通风干燥处。
参考资料:Hi.baidu/=。www.jingda17.net。DI
水浴锅主要适用于各大中院校、科研企事业单位的实验室和化验室,数显电子恒温水浴锅广泛位用于干燥、浓缩,蒸馏、浸渍化学试剂,浸渍药品和生物制品,也可用于水浴恒温加热和其它温度试验,是生物、遗传、病毒、水产、环保、医药、卫生、生化实验室、分析室教育科研的必备工具。对各种化学样品,生物制品进行蒸馏、干燥、浓缩及温渍,是实验室不可缺少的实验器材。
水浴锅工作原理: 恒温水浴锅内水平放置不锈钢管状加热器,水槽的内部放有带孔的铝制搁板。上盖上配有不同口径的组合套圈,可适应不同口径的烧瓶。水浴锅左侧有放水管,恒温水浴锅右侧是电气箱,电气箱前面板上装有温度控制仪表、电源开关。电气箱内有电热管和传感器。该温度控制系统采用了优质电子元件,控温灵敏、性能可靠、使用方便。其工作原理:Cu50传感器将水槽内水的温度转换为电阻值,经过集成放大器的放大、比较后,输出控制信号,有效地控制电加热管的平均加热功率,使水槽内的水保持恒温。
水浴锅操作与维护 1.实验前根据实验要求,在水槽中加入适量的清水或纯化水,至隔板或更高些。然后将实验器皿放置在搁架上,并盖好孔盖。
2.水浴锅应平放在固定平台上,电源电压必须与本箱要求的电压相符,电源插座要采用三孔安全插座,使用前必须接妥地线。
3.接通电源,将电源开关置于ON端,将温度设定-测量开关拨向测量端,绿灯亮,电源正常加热,然后按所需温度转动温度设定旋钮,进行温度的设定,此时LED显示设定的温度值,当设定温度高于水槽水温时仪器开始加热。绿灯亮,加热器开始加热,红灯亮时加热器停止加热,红绿灯交替跳动表示进入恒温状态。若需改变温度,随时转设定旋钮,使用时,LED显示的温度,就是实际所需温度。
4.将温度设定-测量开关拨向测量端,册系统自动测量水槽内试剂温度,此时,LED显示的温度,就是实际所需温度。
主要特点: ●工作室内胆选材不锈钢,有优越的抗腐蚀性能。
●温控精确,数字显示,自动控温。
●操作简便,使用安全。
thermostat water bath
http://www.leicABIosystems.com/cn/%E6%A0%87%E6%9C%AC%E5%88%B6%E5%A4%87/%E5%88%87%E7%89%87/%E6%9F%93%E8%89%B2%E7%9A%84%E5%87%86%E5%A4%87/%E8%AF%A6%E7%BB%86%E4%BB%8B%E7%BB%8D/product/Leica-hi1210/
取下CPU时发现主板掉焊点、塑料排线座损坏,甚至在吹焊CPU时出现短路,更换新CPU或其它BGA封装IC也不能正常工作,其原因是维修人员不了解热风枪的特性所致。
现以850热风枪为例说明如下。
在吹塑料外壳功放时,最好把热风枪的温度调到5.5格,热风枪的风量刻度调到6.5~7格,实际温度是270~280℃,风枪嘴离功放的高度为8cm左右。吹功放的四边(因为金属导热快,锡很快就熔化)热量会很快进入功放的底部,这样就可将功放完好无损地取下。 焊入新功放时应先用风枪给主板加热,加热到主板下面的锡熔化时再放入功放,吹功放的四边即可。
吹CPU时应把热风枪的枪嘴去掉,热风枪的温度调到6格,风量刻度调到7~8格,实际温度是280~290℃,热风枪嘴离CPU的高度为8cm左右。然后用热风枪斜着吹CPU四边,尽量把热风吹进CPU下面,这样即可完好无损地取下CPU。
取下或焊上塑料排线座,注意掌握热风枪的温度和风量即可。 吹焊CPU时常会出现短路,更换新CPU或其它BGA封装IC时有时也会出现短路现象。笔者的经验是在吹焊CPU或其它BGA封装IC时,应对主板BGA IC位置主板下方清洗干净再涂上助焊剂,IC也同样清洗干净,还要注意IC在主板的位置一定要准确,使用热风枪风量要小,温度应为270~280℃。在吹焊IC时还应注意锡球的大小。锡球太大,吹焊时应使IC活动范围小些,这样IC下面的锡球就不容易粘到一起造成短路;若锡球较小,活动范围可大些。
接主板断线或掉点时,可以使用绿油、耐热胶、101、502胶等固定。用胶固定是一个好办法,无论断多少线和掉多少点,即使飞线,也可一次完成。
此外还应注意,主板上不要涂助焊剂,而应在CPU上涂助焊剂。开始接线时要用吸锡线把主板CPU处多余的锡吸净再接线,这样就不会出现凹凸不平的现象,定位更容易。定好位后,焊接时不要用任何工具固定CPU,CPU下面的锡熔化后若有微小的移动,如果能看出来就说明失败,如果在焊接时看不出CPU移动,那就表示成功了。
(ENOHK 伊诺华科 友情提醒)

