Product Specifications:
Item# 115-20: Murine anti-CFP-10 M.tuberculosis Antigen Monoclonal Antibody
Antibody Class: IgG ( Isoptype:ND)
Concentration: See vial
Mass/vial: 100µg
Purity: >95%
Stabilizer: None
Preservative: None
Physical State: Frozen Liquid
Storage: -75°C
Stability: At lea t 24 months s at -75°C
Applications: ELISA, Western ELISA, Immuno precipitation
Description: High affinity murine murine anti-CFP-10 M. tuberculosis antigen-specific monoclonal antibody.
Specificity: Binds to native and recombinant CFP-10 in Elisa and Western Elisa.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 1ug/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml.
Academic articles related to Murine mAb ESAT-6
Novel monoclonal antibodies to ESAT-6 and CFP-10 antigens for ELISA-based diagnosis of pleural tuberculosis.
OBJECTIVE:
To elucidate the potential of monoclonal antibodies (mAbs) of culture filtrate protein 10 (CFP-10) and early secretory antigenic target 6 (ESAT-6) in tuberculosis (TB) diagnosis.
DESIGN:
We generated and characterized monoclonal and polyclonal antibodies against Mycobacterium tuberculosis-specific antigens ESAT-6 and CFP-10 by immunising BALB/c mice with an ESAT-6/CFP-10 fusion protein. Stable hybridoma cell lines were established and mAbs were specifically identified by immunoblotting and immuno precipitation. The mouse mAbs were used to coat plates, and biotin-labelled polyclonal antibodies were used to detect the antigens. One hundred and seventy-three samples of sputum culture supernatants and pleural effusion aspirates have been tested.
RESULTS:
The ESAT-6 enzyme-linked immunosorbent assay (ELISA) detected the culture supernatants and pleural effusion specimens that were positive for M. tuberculosis, but failed to identify M. tuberculosis-positive specimens in the non-M. tuberculosis culture supernatants or control specimens. This yielded a sensitivity of 95.4% and a specificity of 100% for the ESAT-6-specific ELISA. The CFP-10 ELISA presented less satisfactory sensitivity and specificity, of respectively 81.6% and 92.2%. Results showed positive detection rates of ESAT-6 and CFP-10 of 86.8% (33/38) and 76.3% (29/38) for the diagnosis of tuberculous pleural effusion in patients bacteriologically negative for M. tuberculosis culture.
CONCLUSION:
The ESAT-6 and CFP-10 ELISAs incorporating mAbs generated in this study serve as potential tools in the laboratory diagnosis of TB.
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（2）电动离心机如有噪音或机身振动时，应立即切断电源，即时排除故障。
（3）离心管必须对称放入套管中，防止机身振动，若只有一支样品管另外一支要用等质量的水代替。
（4）启动离心机时，应盖上离心机顶盖后，方可慢慢启动。
（5）分离结束后，先关闭离心机，在离心机停止转动后，方可打开离心机盖，取出样品，不可用外力强制其停止运动。
（6）离心时间一般1～2分钟，在此期间，实验者不得离开去做别的事。
靠转筒高速旋转，令里面的液体产生离心力分离或附在筒壁上。常用的衣服脱水机就可以看成是一种离心机。
一般用于煤焦油等脱水脱渣的设备，根据液体中介质的密度不同旋转后产生分层，从而起到分离的效果
离心机机械分离过程主要是物理过程，分离机械分离性能的优劣，与被分离物料的物理性能有极大的关系。如利用沉降原理进行分离的重力沉降或沉降式离心机与固相颗粒的粒径分布、固相密度、颗粒形状以及液相的密度、粘度和表面张力等均密切相关。各种沉降式分离机械的适应范围也依据物料的性质来划分。
步骤/方法1、机器裸露部分涂有防锈油脂，安装前应清除之。待安装完毕，重新涂上润滑油。然后将机器安装在混凝土基础或钢架上。安装过滤机时以两横梁为基础校正水平。
2、然后灌浇混凝土。机器周围要留有余地.考虑安装、拆卸管道以及出滤饼和检修等的方便。按要求接入管道、阀门等。根据您的机型和工艺需要配好管路。
3、按线路容量选好橡套软电缆，外套金属软管，分别接入L1、L2、L3和PEN。试运转；观察油泵电机（风叶从上端往下看）应顺时针旋转。
4、先安装压滤机的滤布，然后再安装滤板，这是比较正常的流程。关于滤布的选择：最好是根据悬浮液的性质对滤饼的要求选择滤布。
5、滤布的性能决定着过滤效果和滤布的使用寿命。滤布的结构：本机型滤布的结构为袖套式滤布。
6、压滤机的头板已被安装固定在压滤机的止推板上；尾板安装固定在压滤机的压紧板上。按要求品质的液压油，经20μm的滤网孔过滤后加入油箱内至视镜上限。
7、启动油泵将油压入液压缸，此时液位应位于视镜中线位。查看过滤机的压力表。表示数值应≤相应款式压滤机插页中所规定的液压工作压力的Mpa数。
8、如查证液压泵系统正常、压力表无误、压力不符合插页规定数值时，可调节压力。调节压力：使泄压压力大于液压工作压力约1Mpa。
9、电接点压力表上限调整至液压工作压力值，下限为液压工作压力减去2Mpa。检查并调整自动型压滤机的传动部分和拉板盒的正确位置。
注意事项（1）液相属易挥发性或易引起爆炸和易燃性物料时，不能用真空过滤，且要求密闭防爆。
（2）腐蚀性较强的物料，对分离机械材料的选择有特殊要求。
（3）固相颗粒硬度较大时，则要求所选分离机械材料的耐磨性好。
（4）被分离物料，无论是固相或液相，若属于贵重物料，则要求回收率高。
（5）固相物料为结晶产品时，要求分离时结晶的破损程度低。这对分离机械的结构及卸料方式、方法都有特殊要求。
普通生化检查分离血清，这个是用低速离心机转速在3000—3500转。一般在3分钟之内就可以了，分析分离效果，可以酌情调节转速和时间，用试验型的那种小的就行
至于管式离心机分离血清生产疫苗，这个有着严格的工艺规范，只能照做，不能随意更改参数
富一阳光与你交流离心机分离机设备相关问题，希望对你有帮助~
