Description
Product Highlights
- Fast – simple protocol for isolation of high quality total RNA, genomic DNA and proteins in as little as 60 minutes
- Efficient – RNA, DNA and protein can be recovered from samples precious or biopsy samples through sequential isolation
- Convenient – an easy-to-use green dye enables easy determination of the phases for improved pipetting accuracy
- Flexible – reagent volume can be scaled for effective extraction of small to large input amounts of cells and tissue
- Consistent – protocol eliminates lysate splitting, thereby improving repeatability of results and increasing yield
Product Description
TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials.
By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications.
TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.
The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.
Applications
- qPCR and RT-qPCR
- End-point PCR and RT-PCR
- Northern/Southern/Western, dot and slot blotting
- Genotyping
- Gene expression analysis
- Transgenic analysis
- Epigenetics
- Cell line characterization
- 2D gel electrophoresis
- Mass spectroscopy

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净水器使用砂滤、活性炭、超滤等技术,能在一定程度上净化水源。能去除泥沙、铁锈,但无法彻底去除水垢、细菌、病毒,属于初级过滤。使用KDF技术的净水器,能去除细菌、病毒,部分去除重金属,但无法去除水垢。
软水机基本都是采用离子交换技术,用钠型阳离子置换水中的钙镁离子(水碱),彻底去除水垢,可以给生活带来很多好处。
纯水机采用反渗透膜过滤技术,核心是微孔过滤,只有水分子和比水分子小的矿物质能通过膜并保留下来供饮用;细菌、病毒、水垢、污染物均不能通过膜,成为浓缩水排出;从而达到纯净水的标准。
主要看你具体的需要,
适合你的才是最好的。
总体上来说,在纯水仪器上,进口品牌中密理博、pall、威立雅都算是国内科研单位采购比较多的,当然,售价也较高,一般为国产品牌的3-5倍,除非硬性规定,或者需要用进口高端设备来提升实验室形象,否则,不建议花冤枉钱,因为目前国内很多纯水品牌在技术上都已经追赶甚至超越了进口品牌。
此外,很多人不了解的是,许多欧美品牌的纯水机,虽然技术成熟,但是都是基于国外的水质而研发,而我国自来水水质事实上比欧美国家的低(欧美国家的自来水可直接饮用),因为存在进口品牌的水机在我国水土不服的现象,甚至其前端处理仍需搭配国产设备,只是很多客户并不知晓,尤其是中央供水系统更是如此。这一点需要注意。
国产纯水品牌的话,鱼龙混杂,这里仅仅介绍几个知名度较高,市场做得比较大的,并简单分析对比:
1、锐思捷:在技术上比较成熟,水机的品质和进口品牌做过很多对比和验证,基本上也足以媲美进口品牌了,产品线包含小型机(10-60L)和大型中央供水系统(200L-5T),价格在中高位置;
2、和泰:主做小型机,中央供水系统较少,价格由近万至7万左右,小型机产品线丰富,在市场营销方面下的力度非常大;
3、力康:也算是中高端国产纯水仪器,同时也做其他实验室设备;
此外还有优普、易普易达等区域性品牌。
菜鸟求救,大虾帮忙看看,感激不尽。
纯水机,跟净水机或者净水器的区别是需要用电(大多数),有一个真空储水罐,纯水机包含了净水机或者净水器的功能和使用材质,过滤更高可达到万分之一微米,因为最重要的是它采用了RO反渗透技术,因为过滤更精细,所以一般的水压是不能顺畅通过的,所以需要用电,电要带动机器本身佩戴的增压泵来给原水打压才能通过。
事实上很多细菌病毒异色异味也只有纯水机能完全的过滤掉。
这只是一些最基本的常识。希望对楼主有帮助吧,本人专业从事净水行业多年,也可以看本人以往的知道回答!
RO逆渗透是一种通过目前国际流行的反渗透等办法,对原水进行过滤处理(物理法)后不添加任何化合物而生产出可供人类直接饮用的纯净水机器(也称为终端净水设备)。
采用水质符合中国卫生部《生活饮用水水质卫生规范》(2001)规定的市政自来水为原水,通过2个活性炭滤芯(1个颗粒活性炭、1个烧结活性炭)1个PPF溶喷滤芯对原水进行预过滤,再对预过滤水施加压力令其通过孔径大小为万分之一微米的RO(反渗透,英文Reverse Osmosis)膜,最后通过材质为果壳(椰壳)的载银活性碳(又名小T33)调节水的酸碱度(使制出的纯净水口感变得甘甜醇美)而生产出纯净水。

