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The PromoCell Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes.
The DC Base Medium does not include cytokines and must therefore be supplemented according to the user’s individual needs.
The DC Generation Medium is a defined and xeno-free formulation for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, this medium is free of all animal-derived components and substances of human origin with Human Serum Albumin as the only exception.
PromoCell DC Media consist of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. Indeed, all DC Media must be supplemented with additional cytokines. The cytokines are included with the DC Generation Media but not with the DC Base Media.
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要看清楚每个真空泵的真空度,选择使用。有必要时,自己测定一下其真空度,若与所写数值有差别时,请告知管理员。
使用时记得加冷阱,有必要时请对所蒸产品进行预处理。
使用结束后,先停止加入,并等体系冷却至室温,再放气,关泵,撤掉冷阱,千万不要弄反顺序。
如发现真空泵管道漏气时,请及时与管理员联系。
若是由于个人操作失误,导致真空泵损坏时,管理员有权停其使用权限,并让其负责维修。
本规定自贴出起生效,请大家遵守以上规定。
装置是这样的:旋转蒸发仪上的冷凝管的水直接流到真空泵里以冷却真空泵然后再从真空泵里流出来.
现在的问题是,我第一天清洗过真空泵之后,第二天再用就发现真空泵里的水已经有股臭味了,有没有什么好的办法可以让它不臭?这样每天都处理的话,太浪费时间了.如果哪位有什么好主意的话,请告知,谢谢!
预冻的时候放在液氮里,拿出来时管上包的膜会脱落,没有膜敞口冻干的话会有影响吗?

