Technical Details
Human FGF-2, accession # P090938, Ala135-Ser288
Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium phosphate, 400 mM sodium chloride, 10 mM glutathione (red.) and 5% mannitol, pH 6.5
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 17.3 kDa | 17.3 kDa |
| Purity | ≥ 95 % as determined by SDS-PAGE | ≥ 97 % as determined by SDS-PAGE and RP-HPLC |
| Activity | ≥ 0.9 x 106 IU/mg, calibrated against NIBSC #90/712 Measured in a cell proliferation assay using a FGF-2-dependent cell line, FBHE | 0.9 – 3 x 106 IU/mg, calibrated against NIBSC #90/712 Measured in a cell proliferation assay using a FGF-2-dependent cell line, FBHE Batch specific activity on CoA |
| Endotoxin level | < 1000 EU/mg | ≤ 50 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.
Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 250 µg/ml reconstituted cytokine solution:
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
