
Type:ChickenIgY
Applications:ICC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Peptidecommontotherat(AAA41119)andhuman(NP_001966)NSE

HumanNeuronspecificenolase(NSE)(EC4.2.1.11)isa47,138daltonprotein(434aminoacids)expressedinneuronsoftheperipheralnervoussystem(PNS)andcentralnervoussystem(CNS).NSEcatalyzestheconversionof2-phospho-Dglycerateintophosphoenolpyruvate,andisanessentialenzymeinenergymetabolisminnervoustissues.
NSEcorrespondstoENO2orenolasegammaandisheavilyexpressedinneuronalcells,developingneuronallineageandneuroendocrinecells. ReleaseofNSEfromdamagedneuronsintoCSFandbloodhasalsobeenusedasabioMarkerofneuronalinjury.
Image:Dissociatedcellculturesofneonatalmousebrains,showingNSE(greenstaining)inneurons.Thesecultureswerecounter-stainedwitharabbitanti-GFAPtolocalizeastrocytes,aswellaswithDAPI(bluestaining)tolocalizenuclei.
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