Type:Protein
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
RecombinantHumanBcl-2producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontainingaminoacids1-218.
Bclisananti-apopticprotein.Ithasbeenimplicatedinanumberofcancers,includingmelanoma,breast,prostate,andlungcarcinomas,aswellasschizophreniaandautoimmunity.Itisalsothoughttobeinvolvedinresistancetoconventionalcancertreatment.Thissupportsarolefordecreasedapoptosisinthepathogenesisofcancer.
Image: BCL2 StructureandSequence.
Applications:
- InputMarkerorpositivecontrol(WesternBlotting).
- Functionstudy(bindingassay).
References:
1.FOWLPOXVIRUSENCODESABCL-2HOMOLOGUETHATPROTECTSCELLSFROMAPOPTOTICDEATHTHROUGHINTERACTIONWITHTHEPRO-APOPTOTICPROTEINBAK. JVirol2007Aug8.
2.TRAILactivatesalysosomalpathwayofapoptosisthatisregulatedbyBcl-2proteins. JBiolChem2007Aug8.
3.Bcl-2smallhairpinRNAsenhancerADIation-inducedapoptosisinA549cells. CellBiolInt2007Jun23.
4.GEA3162,aperoxynitritedonor,inducesBcl-2-sensitive,p53-independentapoptosisinmurinebonemarrowcells. BiochemPharmacol2007Jun23.
5.Bcl-2ProteinFamilyMembers:VersatileRegulatorsofCalciumSignalinginCellSurvivalandApoptosis. AnnuRevPhysiol2007Aug6.
6.Neuroprotectiveeffectofnitroglycerininarodentmodelofischemicstroke:evaluationofbcl-2expression. IntRevNeurobiol2007;82:423-35.
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本人目前需从电鳗放电器官中提取乙酰胆碱受体蛋白,提纯需要的步骤和电鳗器官都可以提供,有没有提供外包提纯服务的公司或者实验室。
NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."

