
Type:Protein
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
RecombinantCNCCisexpressedinE.coliasafull-lengthproteinfusedtoaproprietary16-Kdtag(a6xHistagislocatedattheveryN-terminalend).
Image:CNCCStructureandSequence.
Applications:
RecombinanthumanCNCCcanbeuseddirectlyasapositivecontrolinWesternblotting,ELISA,immunoprecipitationandotherimmunologicalexperiments.TheBIOLOGicalactivityofthisproducthasnotyetbeentested.
References:
1.GeneExpressionsSpecificallyDetectedinMotorNeurons(Dynactin1,EarlyGrowthResponse3,Acetyl-CoATransporter,DeathReceptor5,andCyclinC)DifferentiallyCorrelatetoPathologicMarkersinSporADIcAmyotrophicLateralSclerosis. JNeuropatholExpNeurol2007Jul;66(7):617-627.
2.TwoisoformsofthehumancyclinCgeneareexpresseddifferentiallysuggestingthattheymayhavedistinctfunctions. ActaBiolHung2007Mar;58(1):133-7.
3.OsmosensitivegeneexpressionoftaurinetransporterandcyclinCinembryonicfibroblastcells. AdvExpMedBiol2006;583:49-57.
4.Highlyfrequentalleliclossofchromosome6q16-23inosteosarcoma:involvementofcyclinCinosteosarcoma. IntJMolMed2006Dec;18(6):1153-8.
5.RegulationoftheoxidativestressresponsethroughSlt2p-dependentdestructionofcyclinCinSaccharomycescerevisiae. Genetics2006Mar;172(3):1477-86.
6.[InfluenceofaerosolsontheexpressionofcyclinB1,cyclinCandproliferatingcellnuclearantigeninwoundtissuehealingofburnedratmodels] ZhonghuaWaiKeZaZhi2005Oct1;43(19):1280-3.
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NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."
本人目前需从电鳗放电器官中提取乙酰胆碱受体蛋白,提纯需要的步骤和电鳗器官都可以提供,有没有提供外包提纯服务的公司或者实验室。

