
Specifications
ForremovalofSUMO(smt3)tagsfromfusionproteinsexpressedusingSUMOprosystems(Cat#s:1000A,1000K,1001A,1001K).LifeSensors’industryleADIngSUMOProtease1(Ulp1),ahighlyactiveandrobustrecombinantprotease,cleavesSUMOfromrecombinantfusionproteins.Unlikethrombin,EK,orTEVprotease,whichrecognizeshortlinearsequences,SUMOProtease1recognizesthetertiarystructureofSUMO.Asaresult,SUMOProtease1willnotcleavewithinthefusedproteinofinterest.Bypurchasingthisproduct,thepurchaseragreestocomplywiththetermsofourLimitedUseLabelLicense.Needbulkquantities?Wewouldbehappytoworkoutacustompricethatfitsyourbudget.Pleaseclickheretorequestaquote.
Info
pfam | PeptidaseC-48 |
Molecularweight | 26kDa |
Fusiontag | His6 |
Source | E.coli |
Purity | >95% |
Physicalstate | 5-10U/µLin50mMHepes,pH7.5,150mMNaCl,10%glycerol |
Activity | >105Unit/mg1UofSUMOProtease1cleaves>90%of100ugSUMOProtease1ControlProteinin1hour(30°C) |
Alternatenames | Ulp1core |
Storage/StABIlity | Storeat-80°C,avoidingrepeatedfreeze-thawcycles. Stableforuptooneyearundertheseconditions. |
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算上其他生物24种
大概是记不太清了
NaturalProteinStopsDeadlyHumanBrainCancerInMice
ScientistsfromJohnsHopkinsandfromtheUniversityofMilanhaveeffectivelyproventhattheycaninhibitlethalhumanbraincancersinmiceusingaproteinthatselectivelyinducespositivechangesintheactivityofcellsthatbehavelikecancerstemcells.ThereportispublishedinNature.
Themostcommontypeofbraincancer-glioblastoma-ismarkedbythepresenceofthesestem-cell-likebraincells,which,insteadoftriggeringthereplacementofdamagedcells,formcancertissue.Stemcells,unlikeallothercellsinthebody,arecapableofformingalmostanykindofcellwhentheright"signals"triggertheirdevelopment.
Fortheirtreatmentexperiment,theresearchersreliedonaclassofproteins,bonemorphogenicproteins,thatcauseneuralstem-cell-likeclusterstolosetheirstemcellproperties,whichinturnstopstheirABIlitytodivide.
Firsttheypretreatedhumanglioblastomacellswithbonemorphogenicprotein4(BMP4),theninjectedthesetreatedcellsintomousebrains.Inmiceinjectedwithcellsthatwerenotpretreated,large,invasivecancersgrew.InthemicewithBMP4-treatedcells,nocancersgrewatall.Threetofourmonthsafterinjection,allmicethatgotuntreatedcellsdied,andnearlyallmicewithBMP4-treatedcellswerealive.
Next,thescientistsdeliveredslow-releaseBMP4-containing"beads"directlyintomousebrainswithimplantedglioblastomacells.Micethatgotemptybeadsdevelopedlargemalignanttumorsanddied.MicewithBMP4beadssurvivedmuchlonger,and80percentsurvivedfourmonthsaftercancercellimplants.
"OurideaistotreatpatientswithBMP4orsomethinglikeitrightaftersurgerytoremoveglioblastomainhopesofpreventingtheregrowthofthecancerandimprovingsurvivaltime,"saysAlessandroOlivi,M.D.,directoroftheDivisionofNeurosurgicalOncologyatHopkinsandacontributortothestudy.
OlivisaysclinicalstudiesusingBMP4couldbeginwithinayearand,ifsuccessful,drugtherapiescouldbeavailabletothepublicwithinthreetofouryears.
"ThiswasproofoftheideathatBMPscouldstopglioblastomabydepletingthestem-cell-likepopulationthatfeedsit,"saysHenryBrem,M.D.,chairmanoftheDepartmentofNeurosurgeryatHopkinsandacollaboratorinthestudy."Thisopensexcitingdoorstofutureresearchintotreatmentsandtherapiesforsuchadevastatingdisease."

