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Novus/Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein/25 ug/7754-BH-025
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RecombinantHumanTGF-beta1(HumanCell-expressed)ProteinSummary

DetailsofFunctionality
MeasuredbyitsABIlitytoinhibittheIL-4-dependentproliferationofHT‑2mouseT cells.Tsang, M.et al.(1995)Cytokine7:389.TheED50forthiseffectis0.04-0.2ng/mL.
Source
Humanembryonickidneycell,HEK293-derivedhumanTGF-beta1proteinAla279-Ser390
Accession#
P01137
N-terminalSequence
Ala279
Structure/Form
Disulfide-linkedhomodimer
Protein/PeptideType
RecombinantProteins
Gene
TGFB1
Purity
>95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
EndotoxinNote
<0.01 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
12.8kDa(monomer).Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
11kDa,reducingconditions
Publications
ReadPublicationsusing7754-BHinthefollowingapplications:
  • Bioassay
    4publications

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein.*1mgpacksize(01M)issuppliedasa0.2µmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein.
Purity
>95%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
ReconstitutionInstructions
Reconstituteat100μg/mLinsterile4mMHClcontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanTGF-beta1(HumanCell-expressed)Protein

  • CEDLAP
  • DPD1
  • latency-associatedpeptide
  • TGFbeta1
  • TGFB
  • TGFB1
  • TGF-beta1protein
  • TGFbeta1
  • TGF-beta1
  • TGFbeta
  • TGF-beta-1
  • transforminggrowthfactorbeta-1
  • transforminggrowthfactor,beta1

Background

TGF‑beta1(transforminggrowthfactorbeta1)isoneofthreecloselyrelatedmammalianmembersofthelargeTGF‑betasuperfamilythatshareacharacteristiccystineknotstructure(1‑7).TGF‑beta1,‑2and‑3arehighlypleiotropiccytokinesthatareproposedtoactascellularswitchesthatregulateprocessessuchasimmunefunction,proliferationandepithelial‑mesenchymaltransition(1‑4).EachTGF‑betaisoformhassomenon‑redundantfunctions;forTGF‑beta1,micewithtargeteddeletionshowdefectsinhematopoiesisandendothelialdifferentiation,anddieofoverwhelminginflammation(2).HumanTGF‑beta1CDNAencodesa390aminoacid(aa)precursorthatcontainsa29aasignalpeptideanda361aaproprotein(8).Afurin-likeconvertaseprocessestheproproteintogenerateanN‑terminal249aalatency‑associatedpeptide(LAP)andaC‑terminal112aamatureTGF‑beta1(8,9).Disulfide-linkedhomodimersofLAPandTGF‑beta1remainnon‑covalentlyassociatedaftersecretion,formingthesmalllatentTGF‑beta1complex(8‑10).CovalentlinkageofLAPtooneofthreelatentTGF‑betabindingproteins(LTBPs)createsalargelatentcomplexthatmayinteractwiththeextracellularmatrix(9,10).TGF‑betaisactivatedfromlatencybypathwaysthatincludeactionsoftheproteaseplasmin,matrixmetalloproteases,thrombospondin1andasubsetofintegrins(10).MaturehumanTGF‑beta1shares100%aaidentitywithpig,dogandcowTGF‑beta1,and99%aaidentitywithmouse,ratandhorseTGF‑beta1.Itdemonstratescross‑speciesactivity(1).TGF‑beta1signalingbeginswithhigh‑affinitybindingtoatypeIIser/thrkinasereceptortermedTGF‑betaRII.Thisreceptorthenphosphorylatesandactivatesasecondser/thrkinasereceptor,TGF‑betaRI(alsocalledactivinreceptor‑likekinase(ALK)‑5),oralternatively,ALK‑1.ThiscomplexphosphorylatesandactivatesSmadproteinsthatregulatetranscription(3,11,12).Contributionsoftheaccessoryreceptorsbetaglycan(alsoknownasTGF‑betaRIII)andendoglin,oruseofSmad-independentsignalingpathways,allowfordisparateactionsobservedinresponsetoTGF‑betaindifferentcontexts(11).
  1. Derynck,R.andK.Miyazono(2008)ColdSpringHarborLaboratoryPressp.29.
  2. Dunker,N.andK.Krieglstein(2000)Eur.J.Biochem.267:6982.
  3. Wahl,S.M.(2006)Immunol.Rev.213:213.
  4. Chang,H.etal.(2002)Endocr.Rev.23:787.
  5. Lin,J.S.etal.(2006)Reproduction132:179.
  6. Hinck,A.P.etal.(1996)Biochemistry35:8517.
  7. Mittl,P.R.E.etal.(1996)ProteinSci.5:1261.
  8. Derynck,R.etal.(1985)Nature316:701.
  9. Miyazono,K.etal.(1988)J.Biol.Chem.263:6407.
  10. Oklu,R.andR.Hesketh(2000)Biochem.J.352:601.
  11. deCaestecker,M.etal.(2004)CytokineGrowthFactorRev.15:1.
  12. Zuniga,J.E.etal.(2005)J.Mol.Biol.354:1052.

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天然蛋白质的组成中不含有的氨基酸
和天然蛋白质中不存在的氨基酸两种问答有啥区别,为啥答案就不一样,如果瓜氨酸和同型半胱氨酸在一起怎么办?
我现在在纯化天然蛋白时第一步层析使用的是苯基的疏水层析柱,样品和缓冲液的硫酸铵浓度为0.5M,PH在5.6左右,发现蛋白能结合到柱子上,但是在洗脱时采用梯度洗脱洗不下来,最后用水洗也洗不下来,后来我用少量的0.15M的氢氧化钠洗出一高峰,请问,这是因为我的蛋白结合的太牢吗?该怎样解决呢?
再就是疏水层析和PH值有这么大的关系吗?
谢谢!
gleason分级_123
隐幽羽2018-02-04
如题,最好有详解
请教,天然菌种发酵的蛋白和重组技术发酵的蛋白在质量检测指标上有何区别?谢谢!
人体能产生20种
算上其他生物24种
大概是记不太清了
哪些食物中富含蛋白质 123
滋小味fA3D2018-02-05
见蛋白质的含量(每100克食物)如下:大米7克、面粉9克、黄豆36克、绿豆24克、豆腐7.4克、白菜2克、茄子2.3克、苹果0.4克、花生27克、猪肉9.5克、牛肉20克、人乳1.5克、牛乳3.3克、鸡蛋15克、鲤鱼17克、对虾21克。虽然人乳、牛乳、鸡蛋中的蛋白质含量较低,但它们所含的必需氨基酸量基本上与人体相符,所以营养价值较高,是膳食中最好的食品。
我是研一的新手,导师昨天说要分给我一个比较难的课题,蛋白质分离纯化。我们实验室主要是做微生物生物化学与分子生物学方面的,没有专门搞蛋白分离纯化的,应该是天然蛋白的分离纯化。天然蛋白质的分离纯化比较难,主要指什么方面比较难呢?仅仅蛋白质分离纯化是不是也不够作一篇硕士论文的工作量呢,是不是也应该有后续的工作或者前期的工作呢?蛋白质分离纯化方面的课题对未来就业有何帮助?谢谢大家!十分感激!
从组织中粗提蛋白时加尿素是为了增加蛋白的溶解,是吧?一般用多大浓度呢?

但是尿素是否会导致天然蛋白的失活呢?通过透析除去尿素后蛋白能“复活”吗?
天然蛋白质中不存在的氨基酸是。。。鸟氨酸羟脯氨酸瓜氨酸哪个啊?好象不同版本答案不一样啊
我想做个目的蛋白的单抗,通过基因克隆、表达、纯化来得到目的蛋白,但是因为用的是原核表达系统,缺少翻译后的正常修饰,所以肯定与天然蛋白有区别,这样的话,作出来的单抗就不一定能够识别组织中的天然蛋白了,有什么方法可以减少他们的差异?也就是尽量让单抗可以识别天然蛋白。谢谢!